The epidermal barrier function is dependent on the serine protease CAP1/Prss8

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Type
Article: article from journal or magazin.
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Publications
Institution
Title
The epidermal barrier function is dependent on the serine protease CAP1/Prss8
Journal
Journal of Cell Biology
Author(s)
Leyvraz  C., Charles  R. P., Rubera  I., Guitard  M., Rotman  S., Breiden  B., Sandhoff  K., Hummler  E.
ISSN
0021-9525 (Print)
Publication state
Published
Issued date
08/2005
Volume
170
Number
3
Pages
487-96
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Abstract
Serine proteases are proteolytic enzymes that are involved in the regulation of various physiological processes. We generated mice lacking the membrane-anchored channel-activating serine protease (CAP) 1 (also termed protease serine S1 family member 8 [Prss8] and prostasin) in skin, and these mice died within 60 h after birth. They presented a lower body weight and exhibited severe malformation of the stratum corneum (SC). This aberrant skin development was accompanied by an impaired skin barrier function, as evidenced by dehydration and skin permeability assay and transepidermal water loss measurements leading to rapid, fatal dehydration. Analysis of differentiation markers revealed no major alterations in CAP1/Prss8-deficient skin even though the epidermal deficiency of CAP1/Prss8 expression disturbs SC lipid composition, corneocyte morphogenesis, and the processing of profilaggrin. The examination of tight junction proteins revealed an absence of occludin, which did not prevent the diffusion of subcutaneously injected tracer (approximately 600 D) toward the skin surface. This study shows that CAP1/Prss8 expression in the epidermis is crucial for the epidermal permeability barrier and is, thereby, indispensable for postnatal survival.
Keywords
Animals Cell Differentiation/*physiology Dehydration/genetics/mortality Epidermis/enzymology/pathology/*physiology Intermediate Filament Proteins/biosynthesis Lipid Metabolism Membrane Proteins/metabolism Mice Mice, Knockout Permeability Serine Endopeptidases/genetics/*physiology Skin Abnormalities/genetics/pathology Tight Junctions/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 12:42
Last modification date
20/08/2019 14:25
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