Type I signal peptidase from Leishmania is a target of the immune response in human cutaneous and visceral leishmaniasis.

Details

Serval ID
serval:BIB_6B968DA46DB7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Type I signal peptidase from Leishmania is a target of the immune response in human cutaneous and visceral leishmaniasis.
Journal
Molecular and Biochemical Parasitology
Author(s)
Rafati S., Salmanian A.H., Taheri T., Masina S., Schaff C., Taslimi Y., Fasel N.
ISSN
0166-6851 (Print)
ISSN-L
0166-6851
Publication state
Published
Issued date
2004
Volume
135
Number
1
Pages
13-20
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The gene encoding type I signal peptidase (Lmjsp) has been cloned from Leishmania major. Lmjsp encodes a protein of 180 amino residues with a predicted molecular mass of 20.5 kDa. Comparison of the protein sequence with those of known type I signal peptidases indicates homology in five conserved domains A-E which are known to be important, or essential, for catalytic activity. Southern blot hybridisation analysis indicates that there is a single copy of the Lmjsp gene. A recombinant SPase protein and a synthetic peptide of the L. major signal peptidase were used to examine the presence of specific antibodies in sera from either recovered or active individuals of both cutaneous and visceral leishmaniasis. This evaluation demonstrated that sera from cutaneous and visceral forms of leishmaniasis are highly reactive to both the recombinant and synthetic signal peptidase antigens. Therefore, the Leishmania signal peptidase, albeit localised intracellularly, is a significant target of the Leishmania specific immune response and highlights its potential use for serodiagnosis of cutaneous and visceral leishmaniasis.
Keywords
Adolescent, Amino Acid Sequence, Animals, Antibodies, Protozoan/blood, Catalytic Domain, Child, Child, Preschool, Cloning, Molecular, DNA, Protozoan/chemistry, DNA, Protozoan/isolation & purification, Gene Dosage, Genes, Protozoan, Humans, Infant, Leishmania major/genetics, Leishmania major/immunology, Leishmaniasis, Cutaneous/diagnosis, Leishmaniasis, Cutaneous/immunology, Leishmaniasis, Visceral/diagnosis, Leishmaniasis, Visceral/immunology, Membrane Proteins/biosynthesis, Membrane Proteins/genetics, Molecular Sequence Data, Molecular Weight, Open Reading Frames, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, DNA, Serine Endopeptidases/biosynthesis, Serine Endopeptidases/genetics
Pubmed
Web of science
Create date
24/01/2008 16:02
Last modification date
20/08/2019 15:25
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