Simultaneous determination of plasma levels of fluvoxamine and of the enantiomers of fluoxetine and norfluoxetine by gas chromatography-mass spectrometry.
Details
Serval ID
serval:BIB_6B76DED99984
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Simultaneous determination of plasma levels of fluvoxamine and of the enantiomers of fluoxetine and norfluoxetine by gas chromatography-mass spectrometry.
Journal
Journal of Chromatography. B, Biomedical Applications
ISSN
1572-6495 (Print)
ISSN-L
1572-6495
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
682
Number
2
Pages
265-272
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
A gas chromatographic-mass spectrometric method is presented which allows the simultaneous determination of the plasma concentrations of fluvoxamine and of the enantiomers of fluoxetine and norfluoxetine after derivatization with the chiral reagent, (S)-(-)-N-trifluoroacetylprolyl chloride. No interference was observed from endogenous compounds following the extraction of plasma samples from six different human subjects. The standard curves were linear over a working range of 10 to 750 ng/ml for racemic fluoxetine and norfluoxetine and of 50 to 500 ng/ml for fluvoxamine. Recoveries ranged from 50 to 66% for the three compounds. Intra- and inter-day coefficients of variation ranged from 4 to 10% for fluvoxamine and from 4 to 13% for fluoxetine and norfluoxetine. The limits of quantitation of the method were found to be 2 ng/ml for fluvoxamine and 1 ng/ml for the (R)- and (S)-enantiomers of fluoxetine and norfluoxetine, hence allowing its use for single dose pharmacokinetics. Finally, by using a steeper gradient of temperature, much shorter analysis times are obtained if one is interested in the concentrations of fluvoxamine alone.
Keywords
Fluoxetine/analogs & derivatives, Fluoxetine/blood, Fluvoxamine/blood, Gas Chromatography-Mass Spectrometry/methods, Humans, Reproducibility of Results, Sensitivity and Specificity, Stereoisomerism
Pubmed
Web of science
Create date
01/03/2013 11:44
Last modification date
20/08/2019 15:25