Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response.

Details

Serval ID
serval:BIB_6B6909929545
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response.
Journal
Journal of Histochemistry and Cytochemistry
Author(s)
Kummer J.A., Broekhuizen R., Everett H., Agostini L., Kuijk L., Martinon F., van Bruggen R., Tschopp J.
ISSN
0022-1554 (Print)
ISSN-L
0022-1554
Publication state
Published
Issued date
2007
Volume
55
Number
5
Pages
443-452
Language
english
Abstract
Several autoinflammatory disorders such as Muckle-Wells syndrome are characterized by mutations in the NALP3/cryopyrin gene. NALP3 and NALP1 proteins can assemble to inflammasomes that activate caspase-1, resulting in the processing of pro-inflammatory cytokines IL-1beta and IL-18. The present study was designed to determine which cells and tissues express NALP1 and NALP3. Monoclonal antibodies were developed and their use revealed distinct distribution profiles of NALP1 and NALP3. Granulocytes, monocytes (very weakly), dendritic cells, and B and T cells all express NALP1 and NALP3. Highest levels of NALP1 are found in T cells and Langerhans cells. Furthermore, NALP1 is present in glandular epithelial structures such as stomach, gut, lung, and, surprisingly, in neurons and testis. In contrast to NALP1, NALP3 shows a more restricted tissue distribution with expression mainly in non-keratinizing epithelia in the oropharynx, esophagus, and ectocervix. Moreover, NALP3 expression is found in the urothelial layer in the bladder. Likewise, a difference in subcellular distribution between NALP1 and NALP3 is observed because NALP1 is localized mainly in the nucleus, whereas NALP3 is predominantly cytoplasmic. We propose that the presence of NALP3 in epithelial cells lining the oral and genital tracts allows the rapid sensing of invading pathogens, thereby triggering an innate immune response.
Keywords
Adaptor Proteins, Signal Transducing/biosynthesis, Adaptor Proteins, Signal Transducing/immunology, Antibodies, Monoclonal, Apoptosis Regulatory Proteins/biosynthesis, Apoptosis Regulatory Proteins/immunology, Carrier Proteins/biosynthesis, Carrier Proteins/immunology, Cell Line, Humans, Immunoblotting, Immunohistochemistry, Inflammation/metabolism, Organ Specificity
Pubmed
Web of science
Create date
24/01/2008 16:19
Last modification date
20/08/2019 15:25
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