Phylogenetically closely related pseudomonads isolated from arthropods exhibit differential insect-killing abilities and genetic variations in insecticidal factors.

Details

Serval ID
serval:BIB_6B330AF7F996
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Phylogenetically closely related pseudomonads isolated from arthropods exhibit differential insect-killing abilities and genetic variations in insecticidal factors.
Journal
Environmental microbiology
Author(s)
Vesga P., Augustiny E., Keel C., Maurhofer M., Vacheron J.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Publication state
Published
Issued date
09/2021
Peer-reviewed
Oui
Volume
23
Number
9
Pages
5378-5394
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Strains belonging to the Pseudomonas protegens and Pseudomonas chlororaphis species are able to control soilborne plant pathogens and to kill pest insects by producing virulence factors such as toxins, chitinases, antimicrobials or two-partner secretion systems. Most insecticidal Pseudomonas described so far were isolated from roots or soil. It is unknown whether these bacteria naturally occur in arthropods and how they interact with them. Therefore, we isolated P. protegens and P. chlororaphis from various healthy insects and myriapods, roots and soil collected in an agricultural field and a neighbouring grassland. The isolates were compared for insect killing, pathogen suppression and host colonization abilities. Our results indicate that neither the origin of isolation nor the phylogenetic position mirror the degree of insecticidal activity. Pseudomonas protegens strains appeared homogeneous regarding phylogeny, biocontrol and insecticidal capabilities, whereas P. chlororaphis strains were phylogenetically and phenotypically more heterogenous. A phenotypic and genomic analysis of five closely related P. chlororaphis isolates displaying varying levels of insecticidal activity revealed variations in genes encoding insecticidal factors that may account for the reduced insecticidal activity of certain isolates. Our findings point towards an adaption to insects within closely related pseudomonads and contribute to understand the ecology of insecticidal Pseudomonas.
Pubmed
Web of science
Open Access
Yes
Create date
06/07/2021 11:15
Last modification date
23/10/2021 6:38
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