Tools and techniques to study ligand-receptor interactions and receptor activation by TNF superfamily members.

Details

Serval ID
serval:BIB_6B30FEFF2933
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tools and techniques to study ligand-receptor interactions and receptor activation by TNF superfamily members.
Journal
Methods in Enzymology
Author(s)
Schneider P., Willen L., Smulski C.R.
ISSN
1557-7988 (Electronic)
ISSN-L
0076-6879
Publication state
Published
Issued date
2014
Volume
545
Pages
103-125
Language
english
Abstract
Ligands and receptors of the TNF superfamily are therapeutically relevant targets in a wide range of human diseases. This chapter describes assays based on ELISA, immunoprecipitation, FACS, and reporter cell lines to monitor interactions of tagged receptors and ligands in both soluble and membrane-bound forms using unified detection techniques. A reporter cell assay that is sensitive to ligand oligomerization can identify ligands with high probability of being active on endogenous receptors. Several assays are also suitable to measure the activity of agonist or antagonist antibodies, or to detect interactions with proteoglycans. Finally, self-interaction of membrane-bound receptors can be evidenced using a FRET-based assay. This panel of methods provides a large degree of flexibility to address questions related to the specificity, activation, or inhibition of TNF-TNF receptor interactions in independent assay systems, but does not substitute for further tests in physiologically relevant conditions.
Pubmed
Web of science
Create date
12/09/2014 6:59
Last modification date
20/08/2019 14:25
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