Dose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytes.

Details

Serval ID
serval:BIB_6AEC83C53415
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dose-dependent effects of adiponectin on ADAMTS-9 gene expression in human chondrocytes.
Journal
Bratislavske lekarske listy
Author(s)
Yaykasli K.O., Hatipoglu O.F., Yaykasli E., Kaya E., Ozsahin M., Uslu M.
ISSN
0006-9248 (Print)
ISSN-L
0006-9248
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
118
Number
7
Pages
386-390
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), comprising of 19 members is a family of peptidases. They have several vital functions in physiological and pathological processes in organisms. ADAMTS-9 has aggrecanolytic activity and is responsible for degradation of aggrecan mainly in articular cartilage. It is known that adiponectin is the most abundantly secreted adipokine (adipocytokines), and the characteristics of adiponectin have not been elucidated yet. It was assumed that adiponectin has anti-inflammatory effect before. However, an inflammatory feature of adiponectin was shown in researches. In our study, the effect of adiponectin on ADAMTS-9 gene expression in primary human chondrocytes was investigated.
Primary human chondrocytes were exposed to adiponectin at 1, 4, 8 and 12 µg/ml doses for certain time period. Total RNA was isolated and reverse-transcribed by random primer after incubation. ADAMTS-9 and β-actin genes expression levels were determined using real-time polymerase chain reaction (qRT-PCR).
The highest upregulation of ADAMTS-9 gene expression level was found at 12 µg/ml dose of adiponectin and 48 h incubation.
Adiponectin is the key element in the maintenance of cartilage homeostasis. Similarly, the involvement of adiponectin in articular inflammatory diseases was demonstrated in detail. These findings bring adiponectin into central place in the research to develop adiponectin based new therapy methods for arthritic diseases. Together with these findings, our results suggest that adiponectin may be involved in the degradation of articular cartilage by increasing ADAMTS-9 gene expression (Tab. 1, Fig. 3, Ref. 35).

Keywords
ADAMTS9 Protein/metabolism, Actins/metabolism, Adiponectin/metabolism, Chondrocytes/metabolism, Gene Expression Regulation, Enzymologic, Gene Transfer Techniques, Humans, Mitogen-Activated Protein Kinases/metabolism, Real-Time Polymerase Chain Reaction, ADAMTS-9 human chondrocytes., adiponectin
Pubmed
Web of science
Open Access
Yes
Create date
09/02/2018 12:16
Last modification date
20/08/2019 14:25
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