The alpha(1A/C)- and alpha(1B)-adrenergic receptors are required for physiological cardiac hypertrophy in the double-knockout mouse.

Details

Serval ID
serval:BIB_6AE48502B5DA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The alpha(1A/C)- and alpha(1B)-adrenergic receptors are required for physiological cardiac hypertrophy in the double-knockout mouse.
Journal
Journal of Clinical Investigation
Author(s)
O'Connell T.D., Ishizaka S., Nakamura A., Swigart P.M., Rodrigo M.C., Simpson G.L., Cotecchia S., Rokosh D.G., Grossman W., Foster E., Simpson P.C.
ISSN
0021-9738 (Print)
ISSN-L
0021-9738
Publication state
Published
Issued date
2003
Volume
111
Number
11
Pages
1783-1791
Language
english
Abstract
Catecholamines and alpha(1)-adrenergic receptors (alpha(1)-ARs) cause cardiac hypertrophy in cultured myocytes and transgenic mice, but heart size is normal in single KOs of the main alpha(1)-AR subtypes, alpha(1A/C) and alpha(1B). Here we tested whether alpha(1)-ARs are required for developmental cardiac hypertrophy by generating alpha(1A/C) and alpha(1B) double KO (ABKO) mice, which had no cardiac alpha(1)-AR binding. In male ABKO mice, heart growth after weaning was 40% less than in WT, and the smaller heart was due to smaller myocytes. Body and other organ weights were unchanged, indicating a specific effect on the heart. Blood pressure in ABKO mice was the same as in WT, showing that the smaller heart was not due to decreased load. Contractile function was normal by echocardiography in awake mice, but the smaller heart and a slower heart rate reduced cardiac output. alpha(1)-AR stimulation did not activate extracellular signal-regulated kinase (Erk) and downstream kinases in ABKO myocytes, and basal Erk activity was lower in the intact ABKO heart. In female ABKO mice, heart size was normal, even after ovariectomy. Male ABKO mice had reduced exercise capacity and increased mortality with pressure overload. Thus, alpha(1)-ARs in male mice are required for the physiological hypertrophy of normal postnatal cardiac development and for an adaptive response to cardiac stress.
Keywords
Animals, Blotting, Western, Body Weight, Cells, Cultured, Dose-Response Relationship, Drug, Echocardiography, Female, Genotype, Heart/physiology, Hypertrophy/genetics, MAP Kinase Signaling System, Male, Mice, Mice, Knockout, Mice, Transgenic, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases/metabolism, Muscle Cells/metabolism, Myocardial Contraction, Myocardium/metabolism, Organ Size, Physical Conditioning, Animal, Receptors, Adrenergic, alpha-1/genetics, Receptors, Adrenergic, alpha-1/physiology, Ribonucleases/metabolism, Sex Factors, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 11:05
Last modification date
20/08/2019 14:25
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