Radioimmunoassay of (8-arginine)-vasopressin. II. Application to determination of antidiuretic hormone in urine
Details
Serval ID
serval:BIB_6ABF78F76C5A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Radioimmunoassay of (8-arginine)-vasopressin. II. Application to determination of antidiuretic hormone in urine
Journal
Acta Endocrinologica
ISSN
0001-5598 (Print)
Publication state
Published
Issued date
11/1975
Volume
80
Number
3
Pages
453-64
Notes
Journal Article --- Old month value: Nov
Abstract
A radioimmunoassay for [8-arginine]-vasopressin (AVP), previously described (Czernichow et al. 1975) has been used for the determination of antidiuretic hormone in a 4 ml urine sample. AVP is extracted from acidified urine with a cation exchanger (Amberlite CG 50) with an overall recovery of 72%. The blank value measured in extracted samples of urine was 0.29 pg/ml +/- 0.21 (SEM) and calculated by extrapolation of the regression line of the recovery experiment was 0.49 pg/ml. The coefficient of variation within-assay was 13% and between-assay 18%. Addition of the amounts of AVP found in each specimen of urine voided gave results nearly identical to those of the amounts found in 24 h pool of urine, indicating that the assay was not affected by changes in concentration of the other urinary components during the day. The daily urinary excretion of AVP measured in 34 subjects was found to be 34 ng in 17 women and 70 ng in 17 men, a significant difference. Urinary concentration and excretion rate of AVP rose during thirst test and during Carter-Robbins test performed in 13 healthy subjects. In the latter test it was observed that the women displayed a strikingly more pronounced AVP elevation after the osmolar stimulus than the men. In both sexes a significant correlation was found between AVP excretion rate and plasma osmolality as well as free water clearance. Three cases of complete or incomplete diabetes insipidus and potomania could be clearly differentiated according to the total output of AVP during the thirst test. Extremely high values of AVP were found in the urine of 5 subjects with Schwartz-Bartter syndrome associated with bronchogenic tumours.
Keywords
Arginine Vasopressin/*urine
Diabetes Insipidus/urine
Female
Humans
Hyponatremia/urine
Lung Neoplasms/urine
Male
Osmolar Concentration
Radioimmunoassay/*methods
Sex Factors
Syndrome
Thirst
Vasopressins/*analogs & derivatives/secretion
Pubmed
Web of science
Create date
15/02/2008 17:57
Last modification date
20/08/2019 15:25