Lentiviral-mediated gene transfer to model triplet repeat disorders.

Details

Serval ID
serval:BIB_6AB7F6FE09E4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Lentiviral-mediated gene transfer to model triplet repeat disorders.
Journal
Methods in Molecular Biology
Author(s)
Régulier E., Zala D., Aebischer P., Déglon N.
ISSN
1064-3745 (Print)
ISSN-L
1064-3745
Publication state
Published
Issued date
2004
Volume
277
Pages
199-213
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system as a new strategy in developing animal models of neurodegenerative diseases. To illustrate the approach, procedures for the production of lentiviral vectors encoding polyQ proteins are provided, as well as methods for the determination of viral titers, in vitro infection, and basic protocols for in vivo studies in rodents.
Keywords
Animals, Cell Line, Female, Gene Transfer Techniques, Genetic Diseases, Inborn/genetics, Humans, Immunohistochemistry, Lentivirus/genetics, Pregnancy, Rats, Rats, Sprague-Dawley, Trinucleotide Repeats/genetics
Pubmed
Create date
13/12/2011 16:33
Last modification date
20/08/2019 14:25
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