Hyperpalatable Diet and Physical Exercise Modulate the Expression of the Glial Monocarboxylate Transporters MCT1 and 4.

Details

Serval ID
serval:BIB_6A67AD940DB8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hyperpalatable Diet and Physical Exercise Modulate the Expression of the Glial Monocarboxylate Transporters MCT1 and 4.
Journal
Molecular neurobiology
Author(s)
Portela L.V., Brochier A.W., Haas C.B., de Carvalho A.K., Gnoato J.A., Zimmer E.R., Kalinine E., Pellerin L., Muller A.P.
ISSN
1559-1182 (Electronic)
ISSN-L
0893-7648
Publication state
Published
Issued date
22/09/2016
Peer-reviewed
Oui
Volume
54
Number
8
Pages
5807-5814
Language
english
Notes
Publication types: Journal Article

Abstract
Hyperpalatable diets (HP) impair brain metabolism, and regular physical exercise has an apparent opposite effect. Here, we combined a prior long-term exposure to HP diet followed by physical exercise and evaluated the impact on some neuroenergetic components and on cognitive performance. We assessed the extracellular lactate concentration, expression of monocarboxylate transporters (MCTs), pyruvate dehydrogenase (PDH), and mitochondrial function in the hippocampus. Male C57BL/6J mice were fed 4 months with HP or a control diet. Subsequently, they were divided in the following groups: control diet sedentary (CDS), control diet exercise (CDE), HP diet sedentary (HPS), and HP diet exercise (HPE) (n = 15 per group) and were engaged for an additional 30-day period of voluntary exercise and HP diet. Relative to the control situation, exercise increased MCT1, MCT4, and PDH protein levels, while the HP diet increased MCT1 and MCT4 protein levels. The production of hydrogen peroxide (H2O2) and the mitochondrial membrane potential (∆Ѱm) stimulated by succinate in hippocampal homogenates were not significantly different between groups. ADP phosphorylation and the maximal respiratory rate induced by FCCP showed similar responses between groups, implying a normal mitochondrial function. Also, extracellular brain lactate levels were increased in the HPE group compared to other groups soon after performing the Y-maze task. However, such enhanced lactate levels were not associated with improved memory performance. In summary, hippocampal protein expression levels of MCT1 and 4 were increased by physical exercise and HP diet, whereas PDH was only increased by exercise. These observations indicate that a hippocampal metabolic reprogramming takes place in response to these environmental factors.

Keywords
Hyperpalatable diet, Lactate, Mitochondrial function, Monocarboxylate transporters, Physical exercise
Pubmed
Web of science
Create date
29/09/2017 8:32
Last modification date
20/08/2019 14:25
Usage data