Involvement of nitric oxide in the vasodilatory response to parathyroid hormone-related peptide in the isolated rabbit kidney.

Details

Serval ID
serval:BIB_6A17615BA766
Type
Article: article from journal or magazin.
Collection
Publications
Title
Involvement of nitric oxide in the vasodilatory response to parathyroid hormone-related peptide in the isolated rabbit kidney.
Journal
Clinical Science
Author(s)
Simeoni U., Massfelder T., Saussine C., Judes C., Geisert J., Helwig J.J.
ISSN
0143-5221 (Print)
ISSN-L
0143-5221
Publication state
Published
Issued date
1994
Peer-reviewed
Oui
Volume
86
Number
3
Pages
245-249
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
The present study was designed to explore the role of NO derived from L-arginine in the vasodilatory response to synthetic human parathyroid hormone-related peptide-(1-34) in the isolated rabbit kidney perfused in the presence of indomethacin (10 mumol/l) and preconstricted with noradrenaline (7.2 nmol/min).
Under control conditions, bolus administrations of acetylcholine (10 mumol/l), an NO-dependent renal vasodilator, verapamil (0.1 mmol/l), an NO-independent renal vasodilator, and parathyroid hormone-related peptide (87 nmol/l) decreased the preconstriction pressure, by 31%, 71% and 43%, respectively.
Bolus administration of 100 mumol/l NG-nitro-L-arginine-methyl ester caused a 20% increment in the perfusion pressure of the noradrenaline-preconstricted kidney. NG-nitro-L-arginine methyl ester inhibited the vasodilatory effect of acetylcholine and parathyroid hormone-related peptide, by 68% and 44%, respectively, but did not alter the verapamil-induced vasodilatation.
Unlike L-arginine, the bolus administration of 1 mumol/l of a mono-substituted L-arginine derivative, N-alpha-benzoyl-L-arginine ethyl ester, durable decreased the noradrenaline/NG-nitro-L-arginine methyl ester-induced preconstriction by 57%.
Both L-arginine and N-alpha-benzoyl-L-arginine ethyl ester effectively reversed the inhibition induced by NG-nitro-L-arginine methyl ester on the vasodilatation elicited by acetylcholine and parathyroid hormone-related peptide.
In conclusion, the formation of NO from L-arginine contributes a substantial part to the vasodilatory action of parathyroid hormone-related peptide. Therefore, parathyroid hormone-related peptide appears to have a place among the renal haemodynamically active substances, whose vasodilatory actions are tuned by NO.
Keywords
Acetylcholine/pharmacology, Animals, Arginine/analogs & derivatives, Arginine/pharmacology, Female, Kidney/drug effects, Male, NG-Nitroarginine Methyl Ester, Nitric Oxide/metabolism, Organ Culture Techniques, Parathyroid Hormone/pharmacology, Parathyroid Hormone-Related Protein, Perfusion, Proteins/pharmacology, Rabbits, Vasodilation/drug effects, Vasodilation/physiology, Verapamil/pharmacology
Pubmed
Web of science
Create date
22/02/2015 11:18
Last modification date
20/08/2019 14:24
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