Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype.

Details

Serval ID
serval:BIB_69F86148EC7D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype.
Journal
Journal of Biological Chemistry
Author(s)
Schwinn D.A., Lomasney J.W., Lorenz W., Szklut P.J., Fremeau R.T., Yang-Feng T.L., Caron M.G., Lefkowitz R.J., Cotecchia S.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Publication state
Published
Issued date
1990
Peer-reviewed
Oui
Volume
265
Number
14
Pages
8183-8189
Language
english
Abstract
A novel alpha 1-adrenergic receptor subtype has been cloned from a bovine brain cDNA library. The deduced amino acid sequence is that of a 466-residue polypeptide. The structure is similar to that of the other adrenergic receptors as well as the larger family of G protein-coupled receptors that have a presumed seven-membrane-spanning domain topography. The greatest sequence identity of this receptor protein is with the previously cloned hamster alpha 1B-adrenergic receptor being approximately 72% within the presumed membrane-spanning domains. Localization on different human chromosomes provides evidence that the bovine cDNA is distinct from the hamster alpha 1B-adrenergic receptor. The bovine cDNA clone expressed in COS7 cells revealed 10-fold higher affinity for the alpha 1-adrenergic antagonists WB4101 and phentolamine and the agonist oxymetazoline as compared with the alpha 1B receptor, results similar to pharmacologic binding properties described for the alpha 1A receptor. Despite these similarities in pharmacological profiles, the bovine alpha 1-adrenergic receptor is sensitive to inhibition by the alkylating agent chloroethylclonidine unlike the alpha 1A-adrenergic receptor subtype. In addition, a lack of expression in tissues where the alpha 1A subtype exists suggests that this receptor may actually represent a novel alpha 1-adrenergic receptor subtype not previously appreciated by pharmacological criteria.
Keywords
Adrenergic alpha-Agonists/metabolism, Adrenergic alpha-Antagonists/metabolism, Amino Acid Sequence, Animals, Base Sequence, Binding, Competitive, Cattle, Cloning, Molecular, Cricetinae, DNA/genetics, DNA Probes, Gene Expression, Glycosylation, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphorylation, Receptors, Adrenergic, alpha/genetics, Receptors, Adrenergic, alpha/metabolism, Restriction Mapping, Sequence Homology, Nucleic Acid, Transfection
Pubmed
Web of science
Create date
24/01/2008 11:05
Last modification date
20/08/2019 14:24
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