Surface markers of cloned human T cells with helper or suppressor activity on pokeweed mitogen-driven B cell differentiation

Details

Serval ID
serval:BIB_69F48C2F153A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Surface markers of cloned human T cells with helper or suppressor activity on pokeweed mitogen-driven B cell differentiation
Journal
European Journal of Immunology
Author(s)
Mingari  M. C., Melioli  G., Moretta  A., Pantaleo  G., Moretta  L.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
10/1982
Volume
12
Number
10
Pages
900-3
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
Human spleen T cells stimulated with pokeweed mitogen (PWM) were cloned under limiting conditions in microculture systems using interleukin 2 and irradiated autologous cells. Clones were screened for helper or suppressor activity on PWM-dependent B cell differentiation by adding cell aliquots to either isolated B cells and PWM or to mixtures of T and B cells and PWM. Out of 97 clones tested, 14 promoted intense B cell differentiation, as assessed by measurements of secreted IgG, and 6 strongly inhibited B cell maturation induced by spleen T cells. All the selected clones maintained their original activity after short-term clonal expansion; in addition, a similar (helper or suppressor) effect was detected when the total number of plasma cells per well was evaluated. Suppressor clones had no cytolytic activity on autologous T and B cell blasts, K562 cells or antibody-coated L 1210 mouse cells. Nine helper and 6 suppressor clones were analyzed for a battery of surface markers. All the clones were E rosette-positive and expressed HLA-DR (Ia) antigens. Fcmu receptor was present on a single helper clone, whereas Fc gamma receptor was expressed on a suppressor clone only. All but two clones expressed the OKT4+/ OKT8-, a single suppressor clone was OKT8+/OKT4-, whereas coexpression of OKT4 and OKT8 antigens was detected in one helper clone. Thus, the claim that helper T cells are OKT4+/OKT8- and suppressor T cells are OKT4-/OKT8+ is not supported by the analysis of their phenotype at the clonal level.
Keywords
Antibodies, Monoclonal/immunology B-Lymphocytes/*cytology/immunology Cell Separation Clone Cells/immunology Humans Immunoglobulin G/biosynthesis Lymphocyte Activation Phenotype Pokeweed Mitogens/pharmacology Receptors, Antigen, T-Cell/*genetics T-Lymphocytes, Helper-Inducer/*immunology T-Lymphocytes, Regulatory/*immunology
Pubmed
Web of science
Create date
25/01/2008 15:14
Last modification date
20/08/2019 14:24
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