Reduced beta-cell glucose transporter in new onset diabetic BB rats.

Details

Serval ID
serval:BIB_69C75016D6CE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Reduced beta-cell glucose transporter in new onset diabetic BB rats.
Journal
Journal of Clinical Investigation
Author(s)
Orci L., Unger R.H., Ravazzola M., Ogawa A., Komiya I., Baetens D., Lodish H.F., Thorens B.
ISSN
0021-9738
Publication state
Published
Issued date
11/1990
Peer-reviewed
Oui
Volume
86
Number
5
Pages
1615-1622
Language
english
Abstract
Previous studies from our laboratories have suggested a defect in glucose transport in islets isolated from BB rats on the first day of overt diabetes. To quantitate by immunostaining the glucose transporter of beta-cells (GLUT-2) before and at the onset of autoimmune diabetes we employed an antibody to its COOH-terminal octapeptide. On the first day of overt diabetes, defined as the day the daily blood glucose first reached 200 mg/dl, the volume density ratio of GLUT-2-positive to insulin-positive beta-cells was only 0.48 +/- 0.06, compared to 0.91 +/- 0.02 in age-matched nondiabetic diabetes-resistant controls (P less than 0.001). In age-matched nondiabetic diabetes-prone rats, most of which would have become diabetic, the ratio was 0.85 +/- 0.02, also less than the controls (P less than 0.05). Protein A-gold labeling of GLUT-2 in beta-cells of day 1 diabetic rats revealed 2.17 +/- 0.16 gold particles per micrometer length of microvillar plasma membranes compared to 3.91 +/- 0.14 in controls (P less than 0.001) and 2.87 +/- 0.24 in the nondiabetic diabetes-prone rats (P less than 0.02). Reduction in GLUT-2 correlates temporally with and may contribute to the loss of glucose-stimulated insulin secretion that precedes profound beta-cell depletion of autoimmune diabetes.
Keywords
Animals, B-Lymphocytes, Diabetes Mellitus, Experimental, Fluorescent Antibody Technique, Glucose, Immunohistochemistry, Insulin, Male, Microscopy, Electron, Monosaccharide Transport Proteins, Rats, Rats, Inbred Strains
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:41
Last modification date
20/08/2019 14:24
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