Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_69B2A223B76D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa.
Journal
Microbiology
Author(s)
Michel L., Bachelard A., Reimmann C.
ISSN
1350-0872[print], 1350-0872[linking]
Publication state
Published
Issued date
2007
Volume
153
Number
Pt 5
Pages
1508-1518
Language
english
Abstract
In response to iron starvation, Pseudomonas aeruginosa produces the siderophore pyochelin. When secreted to the extracellular environment, pyochelin chelates iron and transports it to the bacterial cytoplasm via its specific outer-membrane receptor FptA and the inner-membrane permease FptX. Exogenously added pyochelin also acts as a signal which induces the expression of the pyochelin biosynthesis and uptake genes by activating PchR, a cytoplasmic regulatory protein of the AraC/XylS family. The importance of ferripyochelin uptake genes in this regulation was evaluated. The fptA and fptX genes were shown to be part of the fptABCX ferripyochelin transport operon, which is conserved in Burkholderia sp. and Rhodospirillum rubrum. The fptB and fptC genes were found to be dispensable for utilization of pyochelin as an iron source, for signalling and for pyochelin production. By contrast, mutations in fptA and fptX not only interfered with pyochelin utilization, but also affected signalling and diminished siderophore production. It is concluded from this that pyochelin-mediated signalling operates to a large extent via the ferripyochelin transport system.
Keywords
Bacterial Outer Membrane Proteins/genetics, Bacterial Outer Membrane Proteins/physiology, Bacterial Proteins/biosynthesis, Conserved Sequence, DNA-Binding Proteins/biosynthesis, Gene Deletion, Gene Expression Regulation, Bacterial/physiology, Genes, Bacterial, Membrane Transport Proteins/genetics, Membrane Transport Proteins/physiology, Operon, Phenols/metabolism, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/physiology, Receptors, Cell Surface/genetics, Receptors, Cell Surface/physiology, Signal Transduction/physiology, Transcription Factors/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:00
Last modification date
20/08/2019 14:24
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