Biphasic lung injury during Streptococcus pneumoniae infection in a murine model.

Details

Serval ID
serval:BIB_697FEA47865B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biphasic lung injury during Streptococcus pneumoniae infection in a murine model.
Journal
Medecine et maladies infectieuses
Author(s)
Prevotat A., Rouyer C., Gosset P., Kipnis E., Faure K., Guery B.
ISSN
1769-6690 (Electronic)
ISSN-L
0399-077X
Publication state
Published
Issued date
03/2018
Peer-reviewed
Oui
Volume
48
Number
2
Pages
103-113
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Streptococcus pneumoniae is the leading cause of community-acquired pneumonia. We aimed to analyze the epithelial response to S. pneumoniae-induced lung injury.
Using an in vitro model with 16HBE cells and experimental in vivo murine model of acute lung injury, we analyzed the epithelial response to S. pneumoniae. Lung epithelial cell monolayers were exposed to S. pneumoniae and permeability was assessed by transepithelial resistance (TER) measurement and organization and expression of junction proteins. Functional consequences were studied with an in vivo murine model measuring alveolar permeability, distal alveolar fluid clearance (DAFC), and the alveolar inflammatory response.
In vitro, S. pneumoniae induced a dose-dependent decrease in transepithelial resistance, which was associated with significant modifications in the organization of junction proteins assessed by immunofluorescence staining and expression after 6hours of exposure. In vivo, S. pneumoniae induced a transient increase in alveolar permeability with an adequate increase in DAFC 6hours post infection. In a second phase, a permanent increased permeability was associated with a major decrease in DAFC.
Overall, the epithelial response to S. pneumoniae followed a biphasic pattern with an initial reversible increase in permeability related to the alteration of tight and adherens junctions and a second phase associated with an epithelial injury with a major increase in permeability with a decreased DAFC reflecting an injured alveolar capillary barrier.
Keywords
Acute Lung Injury/microbiology, Animals, Disease Models, Animal, Female, Mice, Mice, Inbred C57BL, Pneumonia, Pneumococcal/complications, Acute lung injury, Agression pulmonaire aiguë, Pneumocoque, Pneumonia, Pneumonie, Streptococcus pneumoniae
Pubmed
Web of science
Create date
07/12/2017 22:36
Last modification date
20/08/2019 15:24
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