7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.

Details

Serval ID
serval:BIB_6978220BE26D
Type
Article: article from journal or magazin.
Collection
Publications
Title
7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.
Journal
Nature
Author(s)
Nguyen V.T., Kiss T., Michels A.A., Bensaude O.
ISSN
0028-0836 (Print)
ISSN-L
0028-0836
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
414
Number
6861
Pages
322-325
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The transcription of eukaryotic protein-coding genes involves complex regulation of RNA polymerase (Pol) II activity in response to physiological conditions and developmental cues. One element of this regulation involves phosphorylation of the carboxy-terminal domain (CTD) of the largest polymerase subunit by a transcription elongation factor, P-TEFb, which comprises the kinase CDK9 and cyclin T1 or T2 (ref. 1). Here we report that in human HeLa cells more than half of the P-TEFb is sequestered in larger complexes that also contain 7SK RNA, an abundant, small nuclear RNA (snRNA) of hitherto unknown function. P-TEFb and 7SK associate in a specific and reversible manner. In contrast to the smaller P-TEFb complexes, which have a high kinase activity, the larger 7SK/P-TEFb complexes show very weak kinase activity. Inhibition of cellular transcription by chemical agents or ultraviolet irradiation trigger the complete disruption of the P-TEFb/7SK complex, and enhance CDK9 activity. The transcription-dependent interaction of P-TEFb with 7SK may therefore contribute to an important feedback loop modulating the activity of RNA Pol II.
Keywords
Binding Sites, Cyclin T, Cyclin-Dependent Kinase 9, Cyclin-Dependent Kinases/antagonists & inhibitors, Cyclin-Dependent Kinases/metabolism, Cyclins/antagonists & inhibitors, Cyclins/metabolism, DNA, Viral, Enzyme Inhibitors, Gene Expression Regulation, HIV/genetics, HeLa Cells, Humans, Positive Transcriptional Elongation Factor B, Promoter Regions, Genetic, Protein Kinases/metabolism, Protein-Serine-Threonine Kinases/antagonists & inhibitors, Protein-Serine-Threonine Kinases/chemistry, RNA Polymerase II/metabolism, RNA, Small Nuclear/metabolism
Pubmed
Web of science
Create date
28/07/2008 16:04
Last modification date
20/08/2019 14:24
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