hnRNP proteins and B23 are the major proteins of the internal nuclear matrix of HeLa S3 cells.

Details

Serval ID
serval:BIB_693ACE10957E
Type
Article: article from journal or magazin.
Collection
Publications
Title
hnRNP proteins and B23 are the major proteins of the internal nuclear matrix of HeLa S3 cells.
Journal
Journal of Cellular Biochemistry
Author(s)
Mattern K.A., Humbel B.M., Muijsers A.O., de Jong L., van Driel R.
ISSN
0730-2312 (Print)
ISSN-L
0730-2312
Publication state
Published
Issued date
1996
Volume
62
Number
2
Pages
275-289
Language
english
Abstract
The nuclear matrix is the structure that persists after removal of chromatin and loosely bound components from the nucleus. It consists of a peripheral lamina-pore complex and an intricate internal fibrogranular structure. Little is known about the molecular structure of this proteinaceous internal network. Our aim is to identify the major proteins of the internal nuclear matrix of HeLa 53 cells. To this end, a cell fraction containing the internal fibrogranular structure was compared with one from which this structure had been selectively dissociated. Protein compositions were quantitatively analyzed after high-resolution two-dimensional gel electrophoresis. We have identified the 21 most abundant polypeptides that are present exclusively in the internal nuclear matrix. Sixteen of these proteins are heterogeneous nuclear ribonucleoprotein (hnRNP) proteins. B23 (numatrin) is another abundant protein of the internal nuclear matrix. Our results show that most of the quantitatively major polypeptides of the internal nuclear matrix are proteins involved in RNA metabolism, including packaging and transport of RNA.
Keywords
Cell Fractionation, Cell Nucleus/chemistry, Electrophoresis, Gel, Two-Dimensional, HeLa Cells/chemistry, Heterogeneous-Nuclear Ribonucleoproteins, Humans, Nuclear Matrix/chemistry, Nuclear Matrix/ultrastructure, Nuclear Proteins/analysis, Ribonucleoproteins/analysis, Vimentin/analysis
Pubmed
Web of science
Create date
18/10/2012 13:56
Last modification date
20/08/2019 14:24
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