Generation of a syngeneic mouse model to study the effects of vascular endothelial growth factor in ovarian carcinoma.

Details

Serval ID
serval:BIB_692DA4D693EE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Generation of a syngeneic mouse model to study the effects of vascular endothelial growth factor in ovarian carcinoma.
Journal
American Journal of Pathology
Author(s)
Zhang L., Yang N., Garcia J.R., Mohamed A., Benencia F., Rubin S.C., Allman D., Coukos G.
ISSN
0002-9440 (Print)
ISSN-L
0002-9440
Publication state
Published
Issued date
2002
Volume
161
Number
6
Pages
2295-2309
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Vascular endothelial growth factor (VEGF) performs multifaceted functions in the tumor microenvironment promoting angiogenesis, suppressing anti-tumor immune response, and possibly exerting autocrine functions on tumor cells. However, appropriate syngeneic animal models for in vivo studies are lacking. Using retroviral transfection and fluorescence-activated cell sorting, we generated a C57BL6 murine ovarian carcinoma cell line that stably overexpresses the murine VEGF164 isoform and the enhanced green fluorescent protein. VEGF164 overexpression dramatically accelerated tumor growth and ascites formation, significantly enhanced tumor angiogenesis, and substantially promoted the survival of tumor cells in vivo. In vitro, VEGF164 overexpression significantly enhanced cell survival after growth factor withdrawal and conferred resistance to apoptosis induced by cis-platin through an autocrine mechanism. VEGF/green fluorescent protein-expressing tumors were not recognized by the adaptive immune system. After vaccination, a specific anti-tumor T-cell response was detected, but tumor growth was not inhibited. This engineered murine carcinoma model should prove useful in the investigation of the role of VEGF in modulating the tumor microenvironment and affecting the complex interactions among angiogenesis mechanisms, anti-tumor immune mechanisms, and tumor cell behavior at the natural state or during therapy in ovarian carcinoma.
Keywords
Animals, Antineoplastic Agents/metabolism, Apoptosis/physiology, Ascites/metabolism, Cancer Vaccines/immunology, Cancer Vaccines/metabolism, Carcinoma/immunology, Carcinoma/metabolism, Cell Separation, Cell Transplantation, Cisplatin/pharmacology, Disease Models, Animal, Endothelial Growth Factors/genetics, Endothelial Growth Factors/immunology, Female, Flow Cytometry, Green Fluorescent Proteins, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Intercellular Signaling Peptides and Proteins/genetics, Intercellular Signaling Peptides and Proteins/immunology, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Lymphokines/genetics, Lymphokines/immunology, Mice, Mice, Inbred C57BL, Neoplasm Metastasis, Neoplasm Transplantation, Neovascularization, Pathologic, Ovarian Neoplasms/immunology, Ovarian Neoplasms/metabolism, Peritoneal Neoplasms/metabolism, Peritoneal Neoplasms/pathology, Protein Isoforms/genetics, Protein Isoforms/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Tumor Cells, Cultured, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors
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Web of science
Create date
14/10/2014 11:43
Last modification date
20/08/2019 14:24
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