HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.

Details

Serval ID
serval:BIB_692154A823F6
Type
Article: article from journal or magazin.
Collection
Publications
Title
HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.
Journal
Journal of Virology
Author(s)
Wittek R., Menna A., Schümperli D., Stoffel S., Müller H.K., Wyler R.
ISSN
0022-538X[print], 0022-538X[linking]
Publication state
Published
Issued date
09/1977
Volume
23
Number
3
Pages
669-678
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.
Keywords
Base Sequence, DNA Restriction Enzymes/metabolism, DNA, Viral/analysis, Endonucleases/metabolism, Genes, Molecular Weight, Nucleic Acid Conformation, Nucleic Acid Hybridization, Poxviridae/analysis, Vaccinia virus/analysis
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:24
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