NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Muller cells

Details

Serval ID
serval:BIB_68D17F145DBD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Muller cells
Journal
Neurochemistry International
Author(s)
Alvaro  A. R., Rosmaninho-Salgado  J., Santiago  A. R., Martins  J., Aveleira  C., Santos  P. F., Pereira  T., Gouveia  D., Carvalho  A. L., Grouzmann  E., Ambrosio  A. F., Cavadas  C.
ISSN
0197-0186 (Print)
Publication state
Published
Issued date
04/2007
Volume
50
Number
5
Pages
757-63
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Muller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y(1), Y(2), Y(4) and Y(5) receptors. Retina endothelial cells express all NPY receptors except NPY Y(5) receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Muller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.
Keywords
Animals Cell Line Cells, Cultured Endothelial Cells/*chemistry Microglia/*chemistry Neuropeptide Y/*analysis/genetics RNA, Messenger Rats Rats, Wistar Receptors, Neuropeptide Y/analysis/genetics Retina/*chemistry/cytology Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Create date
25/01/2008 10:55
Last modification date
20/08/2019 14:23
Usage data