Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. III. Clonal analysis of the syngeneic cytolytic T lymphocyte response

Details

Serval ID
serval:BIB_68C486600F28
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. III. Clonal analysis of the syngeneic cytolytic T lymphocyte response
Journal
European Journal of Immunology
Author(s)
Maryanski  J. L., Van Snick  J., Cerottini  J. C., Boon  T.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
05/1982
Volume
12
Number
5
Pages
401-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
The cytolytic T lymphocyte (CTL) response of syngeneic mice to antigenic variants obtained by mutagenesis of mastocytoma P815 was analyzed at the clonal level. Estimates of the frequency of CTL precursor cells in spleens from mice immunized with P815 variants ranged from 10(-4) to 2 X 10(-3). This frequency could be increased approximately 100 times by stimulating immune spleen cells in mass culture for 6-8 days with the immunizing variant. Specificity analysis of a large number of individual CTL clones demonstrated the existence of two distinct populations of CTL. Some CTL clones lysed exclusively the immunizing variant, while others lysed equally well all P815 targets, but not syngeneic tumor L1210. These results provide direct evidence for the existence of new, individual specificities on P815 variants in addition to a common antigen already present on the original P815 cells. This confirms that a variety of new antigens can be induced by mutagenesis on the same background cell. Stable, highly active CTL clones specific for either individual variant antigens or common P815 antigens could be maintained in long-term culture.
Keywords
Animals Antigens, Neoplasm/genetics/immunology Cell Transformation, Neoplastic/*chemically induced Clone Cells/immunology *Cytotoxicity, Immunologic Epitopes/genetics Hematopoietic Stem Cells/immunology Mast-Cell Sarcoma/classification/genetics/*immunology Mice Mice, Inbred DBA Mutagens/pharmacology T-Lymphocytes/*immunology Variation (Genetics)
Pubmed
Web of science
Create date
28/01/2008 11:14
Last modification date
20/08/2019 14:23
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