A respirometer for investigating oxidative cell metabolism: toward optimization of respiratory studies

Details

Serval ID
serval:BIB_689843CA2E2F
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
A respirometer for investigating oxidative cell metabolism: toward optimization of respiratory studies
Journal
Analytical Biochemistry
Author(s)
Haller  T., Ortner  M., Gnaiger  E.
ISSN
0003-2697
Publication state
Published
Issued date
05/1994
Volume
218
Number
2
Pages
338-42
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 1
Abstract
Oxygen flux measurements are critical at low rates of. respiration and at oxygen tensions below air saturation. Difficulties are primarily due to increased oxygen diffusion and consumption processes which may occur in conventional closed respirometric chambers. To avoid measurement errors encountered with standard equipment, a new type of oxygraph was developed. This instrument provides reliable quantification of oxygen flux in small organisms, tissue samples, cells, isolated mitochondria, and chloroplasts. The instrument is available commercially or can be rebuilt in a well-equipped laboratory workshop.
Keywords
Cells/*metabolism Electrodes Energy Metabolism Mitochondria/*metabolism Oxidation-Reduction Oxygen/*analysis/metabolism *Oxygen Consumption Partial Pressure Physiology/*instrumentation
Pubmed
Web of science
Create date
25/01/2008 16:08
Last modification date
20/08/2019 14:23
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