Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.

Details

Serval ID
serval:BIB_6888DB2531B0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.
Journal
EMBO Journal
Author(s)
Kim W.T., Kim H., Katanaev V.L., Joon Lee S., Ishitani T., Cha B., Han J.K., Jho E.H.
ISSN
1460-2075 (Electronic)
ISSN-L
0261-4189
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
31
Number
16
Pages
3384-3397
Language
english
Abstract
DP1, a dimerization partner protein of the transcription factor E2F, is known to inhibit Wnt/β-catenin signalling along with E2F, although the function of DP1 itself was not well characterized. Here, we present a novel dual regulatory mechanism of Wnt/β-catenin signalling by DP1 independent from E2F. DP1 negatively regulates Wnt/β-catenin signalling by inhibiting Dvl-Axin interaction and by enhancing poly-ubiquitination of β-catenin. In contrast, DP1 positively modulates the signalling upon Wnt stimulation, via increasing cytosolic β-catenin and antagonizing the kinase activity of NLK. In Xenopus embryos, DP1 exerts both positive and negative roles in Wnt/β-catenin signalling during anteroposterior neural patterning. From subcellular localization analyses, we suggest that the dual roles of DP1 in Wnt/β-catenin signalling are endowed by differential nucleocytoplasmic localizations. We propose that these dual functions of DP1 can promote and stabilize biphasic Wnt-on and Wnt-off states in response to a gradual gradient of Wnt/β-catenin signalling to determine differential cell fates.
Keywords
Animals, Body Patterning, Gene Expression Regulation, Developmental, Signal Transduction, Transcription Factor DP1/metabolism, Wnt Proteins/metabolism, Xenopus/embryology, beta Catenin/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
26/10/2012 18:25
Last modification date
20/10/2020 10:11
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