Mitomycin C aqueous humor concentration after photorefractive keratectomy: an experimental study.

Details

Serval ID
serval:BIB_68265CD2CE9D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mitomycin C aqueous humor concentration after photorefractive keratectomy: an experimental study.
Journal
European journal of ophthalmology
Author(s)
Kymionis G.D., Diakonis V.F., Panagopoulou S.I., Grentzelos M.A., Kazakos D.C., Tzatzarakis M.N., Tsatsakis A.M., Pallikaris A.I.
ISSN
1120-6721 (Print)
ISSN-L
1120-6721
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
19
Number
5
Pages
738-742
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
To evaluate mitomycin C (MMC) aqueous humor concentration after photorefractive keratectomy (PRK).
In this experimental study, twenty-four eyes of 12 male pigmented rabbits were divided into 4 groups and studied at the Institute of Vision and Optics, Department of Medicine, University of Crete, Greece. Eyes in groups 1 and 2 underwent PRK to correct -5 diopters (D) in a 6-mm optical zone, while sponges soaked with 0.02% MMC were applied on the exposed corneal stroma for 60 and 120 seconds, respectively. Similarly, eyes in groups 3 and 4 underwent PRK to correct -10 D in a 6-mm optical zone, while sponges soaked with 0.02% MMC were applied on the exposed corneal stroma for 60 and 120 seconds, respectively. Aqueous humor was extracted from all rabbit eyes 10 minutes after MMC application and high-performance liquid chromatography was performed immediately to detect and quantify MMC levels.
The mean aqueous humor concentration of MMC was 0.23+/-0.03 microg/mL, 0.39+/-0.05 microg/mL, 0.28+/-0.04 microg/mL, and 0.52+/-0.16 microg/mL in groups 1, 2, 3, and 4, respectively. The effect of application time and correction on aqueous humor MMC concentration was significant (p<0.0001 and p=0.019), while the exposure time had a greater impact on aqueous humor MMC concentration when compared with the attempted correction.
Both exposure time of MMC on the corneal stroma and the attempted correction was correlated with MMC aqueous humor concentrations.
Keywords
Administration, Topical, Alkylating Agents/administration & dosage, Alkylating Agents/pharmacokinetics, Animals, Aqueous Humor/metabolism, Chromatography, High Pressure Liquid, Corneal Stroma/drug effects, Disease Models, Animal, Male, Mitomycin/administration & dosage, Mitomycin/pharmacokinetics, Myopia/surgery, Photorefractive Keratectomy, Rabbits, Refraction, Ocular, Time Factors
Pubmed
Web of science
Create date
01/10/2019 15:45
Last modification date
06/10/2019 5:26
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