Different sensitivities to pH of ATP-induced currents at four cloned P2X receptors.

Details

Serval ID
serval:BIB_680A4140DEEA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Different sensitivities to pH of ATP-induced currents at four cloned P2X receptors.
Journal
Journal of Neurophysiology
Author(s)
Stoop R., Surprenant A., North R.A.
ISSN
0022-3077 (Print)
ISSN-L
0022-3077
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
78
Number
4
Pages
1837-1840
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
The effect of changing extracellular pH was studied on the currents induced by ATP or alphabeta-methylene-ATP in HEK293 cells transfected with different P2X receptor subunits. In cells expressing P2X1, P2X3, or P2X4 receptors, the effect of ATP was decreased by acidification. In cells expressing P2X2 receptors, acidification increased the ATP-induced current; this effect was also seen in cells expressing heteromeric P2X2 and P2X3 receptors. At P2X2 receptors, acidification caused a leftward shift in the ATP concentration-response curve, without change in maximum; the pKa for this effect was 7.3. At P2X4 receptors, acidification caused a rightward shift in the ATP concentration-response curve, without change in the maximum; the pKa for this effect was 6.8. The pH dependence of the action of ATP should be taken into account in studies of synaptic transmission, and it may provide a further tool to assign molecular identity to P2X receptors expressed by brain neurons.
Keywords
Adenosine Triphosphate/pharmacology, Animals, Cloning, Molecular, Dose-Response Relationship, Drug, Hydrogen-Ion Concentration, Patch-Clamp Techniques, Receptors, Purinergic P2/drug effects, Sensitivity and Specificity, Synaptic Transmission/drug effects, Synaptic Transmission/physiology
Pubmed
Create date
29/10/2013 10:46
Last modification date
20/08/2019 14:23
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