Trehalose lyophilized platelets for wound healing.

Details

Serval ID
serval:BIB_67E343CDD8CD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Trehalose lyophilized platelets for wound healing.
Journal
Wound repair and regeneration
Author(s)
Pietramaggiori G., Kaipainen A., Ho D., Orser C., Pebley W., Rudolph A., Orgill D.P.
ISSN
1067-1927 (Print)
ISSN-L
1067-1927
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
15
Number
2
Pages
213-220
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
Fresh platelet preparations are utilized to treat a wide variety of wounds, although storage limitations and mixed results have hampered their clinical use. We hypothesized that concentrated lyophilized and reconstituted platelet preparations, preserved with trehalose, maintain and possibly enhance fresh platelets' ability to improve wound healing. We studied the ability of a single dose of trehalose lyophilized and reconstituted platelets to enhance wound healing when topically applied on full-thickness wounds in the genetically diabetic mouse. We compared these results with the application of multiple doses of fresh platelet preparations and trehalose lyophilized and reconstituted platelets as well as multiple doses of vascular endothelial growth factor (VEGF) and wounds left untreated. Trehalose lyophilized and reconstituted platelets, in single and multiple applications, multiple applications of fresh platelets and multiple applications of VEGF increased granulation tissue deposition, vascularity, and proliferation when compared with untreated wounds, as assessed by histology and immunohistochemistry. Wounds treated with multiple doses of VEGF and a single dose of freeze-dried platelets reached 90% closure faster than wounds left untreated. A single administration of trehalose lyophilized and reconstituted platelet preparations enhanced diabetic wound healing, therefore representing a promising strategy for the treatment of nonhealing wounds.

Keywords
Administration, Topical, Animals, Blood Platelets/physiology, Cell Proliferation, Enzyme-Linked Immunosorbent Assay, Freeze Drying, Immunohistochemistry, Male, Mice, Mice, Inbred Strains, Models, Animal, Plasma, Platelet Transfusion, Trehalose/pharmacology, Vascular Endothelial Growth Factor A/pharmacology, Wound Healing/physiology
Pubmed
Web of science
Create date
16/01/2018 14:43
Last modification date
20/08/2019 14:23
Usage data