The influence of Bone Morphogenic Protein-2 on the consolidation phase in a distraction osteogenesis model.

Details

Serval ID
serval:BIB_67D79A124C88
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The influence of Bone Morphogenic Protein-2 on the consolidation phase in a distraction osteogenesis model.
Journal
Injury
Author(s)
Lesaichot V., Leperlier D., Viateau V., Richarme D., Petite H., Sailhan F.
ISSN
1879-0267 (Electronic)
ISSN-L
0020-1383
Publication state
Published
Issued date
2011
Volume
42
Number
12
Pages
1460-1466
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
We asked whether locally applied recombinant-Bone Morphogenic Protein-2 (rh-BMP-2) with an absorbable Type I collagen sponge (ACS) carrier could enhance the consolidation phase in a callotasis model. We performed unilateral transverse osteotomy of the tibia in 21 immature male rabbits. After a latency period of 7 days, a 3-weeks distraction was begun at a rate of 0.5mm/12h. At the end of the distraction period (Day 28) animals were randomly divided into three groups and underwent a second surgical procedure: 6 rabbits in Group I (Control group; the callus was exposed and nothing was added), 6 rabbits in Group II (ACS group; receiving the absorbable collagen sponge soaked with saline) and 9 rabbits in Group III (rh-BMP-2/ACS group; receiving the ACS soaked with 100μg/kg of rh-BMP-2, Inductos(®), Medtronic). Starting at Day 28 we assessed quantitative and qualitative radiographic parameters as well as densitometric parameters every two weeks (Days 28, 42, 56, 70 and 84). Animals were sacrificed after 8 weeks of consolidation (Day 84). Qualitative radiographic evaluation revealed hypertrophic calluses in the Group III animals. The rh-BMP-2/ACS also influenced the development of the cortex of the calluses as shown by the modified radiographic patterns in Group III when compared to Groups I and II. Densitometric analysis revealed the bone mineral content (BMC) was significantly higher in the rh-BMP-2/ACS treated animals (Group III).
Keywords
Absorbable Implants, Analysis of Variance, Animals, Bone Density/drug effects, Bone Morphogenetic Protein 2/administration & dosage, Bone Morphogenetic Protein 2/pharmacology, Bone Regeneration/drug effects, Bone Regeneration/physiology, Bony Callus/drug effects, Bony Callus/physiology, Collagen Type I, Disease Models, Animal, Dose-Response Relationship, Drug, Male, Osteogenesis, Distraction, Osteotomy, Rabbits, Random Allocation, Recombinant Proteins/administration & dosage, Recombinant Proteins/pharmacology, Tibia/radiography, Tibia/surgery, Time Factors
Pubmed
Web of science
Create date
21/02/2012 16:44
Last modification date
20/08/2019 15:23
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