Teichoic acids are not required for Streptococcus pneumoniae and Staphylococcus aureus cell walls to trigger the release of tumor necrosis factor by peripheral blood monocytes.

Details

Serval ID
serval:BIB_67B0F27A67E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Teichoic acids are not required for Streptococcus pneumoniae and Staphylococcus aureus cell walls to trigger the release of tumor necrosis factor by peripheral blood monocytes.
Journal
Infection and Immunity
Author(s)
Majcherczyk P.A., Rubli E., Heumann D., Glauser M.P., Moreillon P.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
2003
Volume
71
Number
7
Pages
3707-3713
Language
english
Abstract
In gram-negative bacteria, the outer membrane lipopolysaccharide is the main component triggering cytokine release from peripheral blood mononuclear cells (PBMCs). In gram-positive bacteria, purified walls also induce cytokine release, but stimulation requires 100 times more material. Gram-positive walls are complex megamolecules reassembling distinct structures. Only some of them might be inflammatory, whereas others are not. Teichoic acids (TA) are an important portion (> or =50%) of gram-positive walls. TA directly interact with C3b of complement and the cellular receptor for platelet-activating factor. However, their contribution to wall-induced cytokine-release by PBMCs has not been studied in much detail. In contrast, their membrane-bound lipoteichoic acids (LTA) counterparts were shown to trigger inflammation and synergize with peptidoglycan (PGN) for releasing nitric oxide (NO). This raised the question as to whether TA are also inflammatory. We determined the release of tumor necrosis factor (TNF) by PBMCs exposed to a variety of TA-rich and TA-free wall fragments from Streptococcus pneumoniae and Staphylococcus aureus. TA-rich walls from both organisms induced measurable TNF release at concentrations of 1 microg/ml. Removal of wall-attached TA did not alter this activity. Moreover, purified pneumococcal and staphylococcal TA did not trigger TNF release at concentrations as high as > or =100 microg/ml. In contrast, purified LTA triggered TNF release at 1 microg/ml. PGN-stem peptide oligomers lacking TA or amino-sugars were highly active and triggered TNF release at concentrations as low as 0.01 microg/ml (P. A. Majcherczyk, H. Langen, et al., J. Biol. Chem. 274:12537-12543,1999). Thus, although TA is an important part of gram-positive walls, it did not participate to the TNF-releasing activity of PGN.
Keywords
Cell Wall/chemistry, Cell Wall/physiology, Humans, Lipopolysaccharides/pharmacology, Monocytes/metabolism, Staphylococcus aureus/immunology, Streptococcus pneumoniae/immunology, Teichoic Acids/pharmacology, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:54
Last modification date
20/08/2019 15:23
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