Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats

Details

Serval ID
serval:BIB_676A20EBBD4B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats
Journal
Journal of Clinical Microbiology
Author(s)
Nahimana  A., Cushion  M. T., Blanc  D. S., Hauser  P. M.
ISSN
0095-1137
Publication state
Published
Issued date
12/2001
Peer-reviewed
Oui
Volume
39
Number
12
Pages
4563-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
A rapid method that uses PCR-single-strand conformation polymorphism analysis of the intron of the nuclear 26S rRNA gene was shown to differentiate the two Pneumocystis carinii special forms that infect rats, P. carinii f. sp. carinii and P. carinii f. sp. ratti. The method also provides a means for estimation of the relative abundance of the two special forms in the case of a coinfected rat. The results suggest that the method described will help to further standardize the immunosuppressed rat model of P. carinii infection and, thus, contribute to a better understanding of P. carinii infection in humans.
Keywords
Animals DNA, Fungal/analysis Genes, rRNA/genetics Humans Lung/microbiology Pneumocystis/*classification/*genetics Pneumonia, Pneumocystis/*microbiology Polymerase Chain Reaction/*methods *Polymorphism, Single-Stranded Conformational RNA, Ribosomal/genetics Rats Rats, Long-Evans Rats, Sprague-Dawley Time Factors
Pubmed
Web of science
Create date
29/01/2008 16:20
Last modification date
20/08/2019 15:22
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