Investigation of human papillomavirus in bladder cancer in a series of Tunisian patients.

Details

Serval ID
serval:BIB_6758491F846B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Investigation of human papillomavirus in bladder cancer in a series of Tunisian patients.
Journal
Pathology, research and practice
Author(s)
Ben Selma W., Ziadi S., Ben Gacem R., Amara K., Ksiaa F., Hachana M., Trimeche M.
ISSN
1618-0631 (Electronic)
ISSN-L
0344-0338
Publication state
Published
Issued date
15/11/2010
Peer-reviewed
Oui
Volume
206
Number
11
Pages
740-743
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The association between human papillomavirus (HPV) infection and development of bladder cancer is variable. Furthermore, the prevalence of HPV DNA in bladder carcinoma subtypes varies from study to study. To clarify the impact of HPV infection on the development of bladder carcinoma, we performed a retrospective study on Tunisian patients to determine the status of HPV infection in urothelial carcinoma, squamous cell carcinoma, and adenocarcinoma. A total of 125 formalin-fixed, paraffin-embedded archival tissue specimens of bladder carcinoma were reviewed and classified according to the World Health Organization (WHO) classification of tumors (119 urothelial carcinomas, five squamous carcinomas, and one adenocarcinoma). Anogenital HPV DNA detection was performed using three different polymerase chain reaction (PCR) techniques: the first one used primers pU-2R/pU-1M specific to high-risk oncogenic HPV; the second one used primers PU-2R/PU-31B specific to low-risk oncogenic HPV; and the third one employed consensus primers (E1-547R/E1-350L). No evidence of HPV infection was detected by morphological examination and PCR in any case of bladder carcinoma. Our study shows that the anogenital HPVs investigated are not associated with the pathogenesis of bladder cancer in Tunisia; however, the question of whether other subtypes of HPV contribute to bladder carcinogenesis remains to be clarified.
Keywords
Adenocarcinoma/pathology, Adenocarcinoma/surgery, Adenocarcinoma/virology, Adult, Aged, Aged, 80 and over, Alphapapillomavirus/genetics, Alphapapillomavirus/isolation & purification, Anus Diseases/virology, Carcinoma, Squamous Cell/pathology, Carcinoma, Squamous Cell/surgery, Carcinoma, Squamous Cell/virology, Carcinoma, Transitional Cell/pathology, Carcinoma, Transitional Cell/surgery, Carcinoma, Transitional Cell/virology, DNA, Viral/analysis, Female, Humans, Male, Middle Aged, Papillomavirus Infections/pathology, Papillomavirus Infections/surgery, Papillomavirus Infections/virology, Polymerase Chain Reaction/methods, Retrospective Studies, Tunisia, Urinary Bladder Neoplasms/pathology, Urinary Bladder Neoplasms/surgery, Urinary Bladder Neoplasms/virology, Urothelium
Pubmed
Web of science
Create date
17/10/2023 9:46
Last modification date
20/10/2023 7:10
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