Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group.

Details

Serval ID
serval:BIB_6603
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group.
Journal
European Journal of Cancer
Author(s)
Keilholz U., Willhauck M., Rimoldi D., Brasseur F., Dummer W., Rass K., de Vries T., Blaheta J., Voit C., Lethé B., Burchill S.
ISSN
0959-8049 (Print)
ISSN-L
0959-8049
Publication state
Published
Issued date
1998
Volume
34
Number
5
Pages
750-753
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Reverse transcription-polymerase chain reaction (RT-PCR)-based assays detecting occult neoplastic cells are increasingly being used for the study of tumour dissemination and minimal residual disease. However, different methods are employed by various research groups and the results are heterogenous. We prospectively assessed the results from nine laboratories performing tyrosinase RT-PCR assays for the detection of melanoma cells on a series of blind samples. After complete analysis, the results were compared for sensitivity and specificity. All laboratories reported correct results for cDNA standards. Five laboratories attained acceptable specificity and a sensitivity detecting 10 cells in 10 ml of whole blood. Four laboratories had unacceptable specificity and/or sensitivity. This blind study highlights the difficulty of RT-PCR data interpretation and the need for quality assurance between laboratories. Measures to increase the reliability of RT-PCR assays are proposed, which have to be prospectively evaluated in future studies.
Keywords
DNA, Neoplasm/analysis, Humans, Melanoma/diagnosis, Neoplastic Cells, Circulating, Polymerase Chain Reaction/methods, Polymerase Chain Reaction/standards, Prospective Studies, RNA, Neoplasm/analysis, Sensitivity and Specificity
Pubmed
Web of science
Create date
19/11/2007 12:43
Last modification date
20/08/2019 14:21
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