Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.

Details

Serval ID
serval:BIB_65E6AB1AE38B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
Journal
Nature communications
Author(s)
Korotkova D., Borisyuk A., Guihur A., Bardyn M., Kuttler F., Reymond L., Schuhmacher M., Amen T.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
21/05/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
4314
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegenerative conditions, including Peroxisome Biogenesis Disorders (PBD). Due to the dynamic, tissue-specific, and context-dependent nature of their biogenesis and function, live cell imaging of peroxisomes is essential for studying peroxisome regulation, as well as for the diagnosis of PBD-linked abnormalities. However, the peroxisomal imaging toolkit is lacking in many respects, with no reporters for substrate import, nor cell-permeable probes that could stain dysfunctional peroxisomes. Here we report that the BODIPY-C12 fluorescent fatty acid probe stains functional and dysfunctional peroxisomes in live mammalian cells. We then go on to improve BODIPY-C12, generating peroxisome-specific reagents, PeroxiSPY650 and PeroxiSPY555. These probes combine high peroxisome specificity, bright fluorescence in the red and far-red spectrum, and fast non-cytotoxic staining, making them ideal tools for live cell, whole organism, or tissue imaging of peroxisomes. Finally, we demonstrate that PeroxiSPY enables diagnosis of peroxisome abnormalities in the PBD CRISPR/Cas9 cell models and patient-derived cell lines.
Keywords
Peroxisomes/metabolism, Humans, Fatty Acids/metabolism, Fluorescent Dyes/chemistry, Boron Compounds/chemistry, Peroxisomal Disorders/metabolism, Animals
Pubmed
Open Access
Yes
Create date
24/05/2024 9:46
Last modification date
25/05/2024 7:13
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