Differential localization of two glucose transporter isoforms in rat kidney.

Details

Serval ID
serval:BIB_65DB17B387D7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential localization of two glucose transporter isoforms in rat kidney.
Journal
American Journal of Physiology.
Author(s)
Thorens B., Lodish H.F., Brown D.
ISSN
0363-6143
Publication state
Published
Issued date
12/1990
Volume
259
Number
6 Pt 1
Pages
C286-C294
Language
english
Abstract
The localization of two glucose transporter isoforms was mapped in the rat kidney: the high-Michaelis constant (Km; 15-20 mM) low-affinity "liver" transporter and the low-Km (1-2 mM) high-affinity "erythroid/brain" transporter. Both are basolateral membrane proteins, but the liver transporter was present exclusively in the S1 part of the proximal tubule, whereas the erythroid/brain transporter was expressed at variable levels in different nephron segments. Staining intensity was low in the straight proximal tubule (S3), intermediate in the medullary thin and thick ascending limbs, and highest in connecting segments and collecting ducts. In the collecting duct, the erythroid/brain glucose transporter was expressed at the highest level in intercalated cells; less was present in principal cells. In the papilla, only intercalated cells expressed this transporter isoform. These results suggest specific involvements of each transporter isoform in transepithelial glucose reabsorption by different segments of the proximal tubule. They also indicate that while the liver glucose transporter is present in gluconeogenic cells, there is a good correlation between the level of expression of the erythroid/brain glucose transporter and the glycolytic activity of the different nephron segments.
Keywords
Animals, Brain, Brain Chemistry, Cell Line, Cell Membrane, Fluorescent Antibody Technique, Humans, Kidney, Kidney Cortex, Kidney Medulla, Monosaccharide Transport Proteins, Rats
Pubmed
Create date
24/01/2008 13:41
Last modification date
20/08/2019 14:21
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