Expression of endomembrane calcium pumps in colon and gastric cancer cells. Induction of SERCA3 expression during differentiation.

Details

Serval ID
serval:BIB_65A448E208CD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of endomembrane calcium pumps in colon and gastric cancer cells. Induction of SERCA3 expression during differentiation.
Journal
Journal of Biological Chemistry
Author(s)
Gélébart P., Kovács T., Brouland J.P., van Gorp R., Grossmann J., Rivard N., Panis Y., Martin V., Bredoux R., Enouf J., Papp B.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
277
Number
29
Pages
26310-26320
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Calcium mobilization from the endoplasmic reticulum (ER) into the cytosol is a key component of several signaling networks controlling tumor cell growth, differentiation, or apoptosis. Sarco/endoplasmic reticulum calcium transport ATPases (SERCA-type calcium pumps), enzymes that accumulate calcium in the ER, play an important role in these phenomena. We report that SERCA3 expression is significantly reduced or lost in colon carcinomas when compared with normal colonic epithelial cells, which express this enzyme at a high level. To study the involvement of SERCA enzymes in differentiation, in this work differentiation of colon and gastric cancer cell lines was initiated, and the change in the expression of SERCA isoenzymes as well as intracellular calcium levels were investigated. Treatment of the tumor cells with butyrate or other established differentiation inducing agents resulted in a marked and specific induction of the expression of SERCA3, whereas the expression of the ubiquitous SERCA2 enzymes did not change significantly or was reduced. A similar marked increase in SERCA3 expression was found during spontaneous differentiation of post-confluent Caco-2 cells, and this closely correlated with the induction of other known markers of differentiation. Analysis of the expression of the SERCA3 alternative splice isoforms revealed induction of all three known iso-SERCA3 variants (3a, 3b, and 3c). Butyrate treatment of the KATO-III gastric cancer cells led to higher resting cytosolic calcium concentrations and, in accordance with the lower calcium affinity of SERCA3, to diminished ER calcium content. These data taken together indicate a defect in SERCA3 expression in colon cancers as compared with normal colonic epithelium, show that the calcium homeostasis of the endoplasmic reticulum may be remodeled during cellular differentiation, and indicate that SERCA3 constitutes an interesting new differentiation marker that may prove useful for the analysis of the phenotype of gastrointestinal adenocarcinomas.
Keywords
Adenocarcinoma/enzymology, Alternative Splicing, Animals, Butyrates/pharmacology, Caco-2 Cells, Calcium-Transporting ATPases/biosynthesis, Calcium-Transporting ATPases/genetics, Cell Differentiation, Colonic Neoplasms/enzymology, Humans, Isoenzymes/biosynthesis, Isoenzymes/genetics, Phenotype, Rabbits, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Stomach Neoplasms/enzymology, Tumor Cells, Cultured, Tumor Markers, Biological
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2015 9:32
Last modification date
20/08/2019 14:21
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