Toward dynamic isotopomer analysis in the rat brain in vivo: automatic quantitation of 13C NMR spectra using LCModel.

Details

Serval ID
serval:BIB_65A0112E2B47
Type
Article: article from journal or magazin.
Collection
Publications
Title
Toward dynamic isotopomer analysis in the rat brain in vivo: automatic quantitation of 13C NMR spectra using LCModel.
Journal
NMR in Biomedicine
Author(s)
Henry P.G., Oz G., Provencher S., Gruetter R.
ISSN
0952-3480 (Print)
ISSN-L
0952-3480
Publication state
Published
Issued date
2003
Volume
16
Number
6-7
Pages
400-412
Language
english
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Validation StudiesPublication Status: ppublish
Abstract
The LCModel method was adapted to analyze localized in vivo (13)C NMR spectra obtained from the rat brain in vivo at 9.4 T. Prior knowledge of chemical-shifts, J-coupling constants and J-evolution was included in the analysis. Up to 50 different isotopomer signals corresponding to 10 metabolites were quantified simultaneously in 400 microl volumes in the rat brain in vivo during infusion of [1,6-(13)C(2)]glucose. The analysis remained accurate even at low signal-to-noise ratio of the order of 3:1. The relative distribution of isotopomers in glutamate, glutamine and aspartate determined in vivo in 22 min was in excellent agreement with that measured in brain extracts. Quantitation of time series of (13)C spectra yielded time courses of total (13)C label incorporation into up to 16 carbon positions, as well as time courses of individual isotopomer signals, with a temporal resolution as low as 5 min (dynamic isotopomer analysis). The possibility of measuring in vivo a wealth of information that was hitherto accessible only in extracts is likely to expand the scope of metabolic studies in the intact brain.
Keywords
Algorithms, Animals, Aspartic Acid/analogs & derivatives, Aspartic Acid/metabolism, Brain/metabolism, Carbon Isotopes/diagnostic use, Carbon Isotopes/pharmacokinetics, Culture Techniques, Feasibility Studies, Glucose/administration & dosage, Glucose/metabolism, Glutamic Acid/metabolism, Glutamine/metabolism, Magnetic Resonance Spectroscopy/methods, Metabolic Clearance Rate, Neurons/metabolism, Neurotransmitter Agents/metabolism, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Sensitivity and Specificity, Staining and Labeling/methods, Synaptic Transmission/physiology, gamma-Aminobutyric Acid/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
04/08/2010 15:28
Last modification date
20/08/2019 14:21
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