An adult thymic stromal-cell suspension model for in vitro positive selection.

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Version: Final published version
Serval ID
serval:BIB_6593
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An adult thymic stromal-cell suspension model for in vitro positive selection.
Journal
Developmental Immunology
Author(s)
Chidgey A.P., Pircher H., MacDonald H.R., Boyd R.L.
ISSN
1044-6672
Publication state
Published
Issued date
1998
Volume
6
Number
3-4
Pages
157-170
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Presented here is a cell-suspension model for positive selection using thymocytes from alphabeta-TCR (H-2Db-restricted) transgenic mice specific to the lymphocytic choriomeningitis virus (LCMV) on a nonselecting MHC background (H-2d or TAP-1 -/-), cocultured with freshly isolated adult thymus stromal cells of the selecting MHC type. The thymic stromal cells alone induced positive selection of functional CD4- CD8+ cells whose kinetics and efficiency were enhanced by nominal peptide. Fibroblasts expressing the selecting MHC alone did not induce positive selection; however, together with nonselecting stroma and nominal peptide, there was inefficient positive. These results suggest multiple signaling in positive selection with selection events able to occur on multiple-cell types. The ease with which this model can be manipulated should greatly facilitate the resolution of the mechanisms of positive selection in normal and pathological states.
Keywords
Animals, Antigens, CD/analysis, Antigens, Differentiation, T-Lymphocyte/analysis, CD4-Positive T-Lymphocytes/cytology, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Cell Differentiation, Cells, Cultured, Coculture Techniques, H-2 Antigens/immunology, Hematopoietic Stem Cells/physiology, Lymphocyte Activation, Lymphocytic choriomeningitis virus/immunology, Mice, Mice, Transgenic, Models, Immunological, Peptides/immunology, Receptors, Antigen, T-Cell, alpha-beta/immunology, Receptors, Antigen, T-Cell, alpha-beta/metabolism, Signal Transduction, Stromal Cells/immunology, T-Lymphocyte Subsets/cytology, T-Lymphocyte Subsets/immunology, Thymus Gland/cytology, Thymus Gland/growth &amp, development
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Create date
19/11/2007 12:43
Last modification date
20/08/2019 14:21
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