Role of complement-derived and bacterial formylpeptide chemotactic factors in the in vivo migration of neutrophils in experimental Escherichia coli pyelonephritis in rats

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Serval ID
serval:BIB_657821773479
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Role of complement-derived and bacterial formylpeptide chemotactic factors in the in vivo migration of neutrophils in experimental Escherichia coli pyelonephritis in rats
Journal
Journal of Infectious Diseases
Author(s)
Meylan  P. R., Glauser  M. P.
ISSN
0022-1899 (Print)
Publication state
Published
Issued date
05/1989
Volume
159
Number
5
Pages
959-65
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
In experimental Escherichia coli pyelonephritis, the bacterial multiplication in the kidney parenchyma triggers a burst of neutrophil extravascular migration, as measured by the myeloperoxidase (MPO) activity in the kidney, a marker for tissue neutrophil infiltration. To test the mechanisms of in vivo neutrophil migration, pyelonephritis was surgically induced in rats that were then either complement-depleted with cobra venom factor (CVF), resulting in a profound hypocomplementemia for 72 h after inoculation, or treated with phenylbutazone (PB), a competitive antagonist of bacterial chemotactic formylpeptides. Compared to controls, CVF- and PB-treated animals killed when the neutrophil infiltration started (32 h) had a significantly reduced neutrophil infiltration, as measured by kidney MPO activity. This effect disappeared in animals killed 72 h after surgery, when neutrophil infiltration peaked. These data suggest that redundant chemotactic mechanisms triggered neutrophil migration. Inhibiting one of these mechanisms only transiently delayed neutrophil migration but did not affect the peak infiltration.
Keywords
Animals Cell Migration Inhibition Cell Movement Chemotactic Factors/*immunology Cobra Venoms Complement C3/immunology Complement C5/immunology Escherichia coli Infections/*immunology Kidney/enzymology Neutrophils/*immunology Peroxidase/analysis Pyelonephritis/*immunology Random Allocation Rats
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:33
Last modification date
01/10/2019 7:18
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