Evidence of stage-specific element for germ-line transcription of the TCR alpha gene located upstream of J alpha49 locus.

Details

Serval ID
serval:BIB_6577
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evidence of stage-specific element for germ-line transcription of the TCR alpha gene located upstream of J alpha49 locus.
Journal
European Journal of Immunology
Author(s)
Hozumi K., Tanaka Y., Sato T., Wilson A., Habu S.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
1998
Volume
28
Number
4
Pages
1368-1378
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
T cell receptor (TCR) genes are rearranged and expressed in an ordered manner during T cell development. The basic mechanism regulating this stepwise DNA alteration is poorly understood. To address this issue, we explored the presence of a stage-specific element for germ-line transcription of the TCR alpha gene which is closely associated with gene rearrangement. First, germ-line transcription of the TCR alpha gene including the first segment of the J alpha locus, J alpha49, was delayed compared to that of the TCR beta gene in both normal and TCR-transgenic (Tg) mice. Furthermore, expression of this transcript could be induced by CD3epsilon-mediated signals in recombination-activating gene (RAG)-2-deficient mice. In TCR-Tg mice, the endogenous J alpha49 germ-line transcript could not yet be observed at the CD25+ double-negative (DN) stage when the TCR alpha transgene was expressed. Of immature T cell hybridomas derived from either scid thymocytes (CD25+ DN) or immature CD8-single positive (ISP) thymocytes, only the latter hybridoma expressed the J alpha49 germ-line transcript. These data indicate that the J alpha49 germ-line transcription occurs only at a specific developmental stage. Second, to determine which elements may be regulating stage specificity, we performed transient transfection analysis with a reporter gene and demonstrated that the upstream region of the J alpha49 locus possesses promoter activity in correlation with germ-line transcription in ISP-derived but not in SCID-derived hybridomas. These results indicate that the expression of TCR alpha germ-line transcripts is regulated in a stage-specific manner by a cis-element located within the J alpha locus.
Keywords
Animals, Cell Differentiation/genetics, Cell Differentiation/immunology, Gene Rearrangement, T-Lymphocyte, Gene Transfer Techniques, Mice, Mice, Inbred C57BL, Promoter Regions, Genetic, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology, T-Lymphocytes/immunology, Transcription, Genetic
Pubmed
Web of science
Create date
19/11/2007 12:43
Last modification date
20/08/2019 14:21
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