Comparison of Whole Genome (wg-) and Core Genome (cg-) MLST (BioNumerics<sup>TM</sup>) Versus SNP Variant Calling for Epidemiological Investigation of Pseudomonas aeruginosa.

Details

Ressource 1Download: Blanc_2020_Front.Micro_Comparison of wg-and cg-MLST versus SNP Variant Calling for Epidemiological Investigation of Paeruginosa.pdf (1476.50 [Ko])
State: Public
Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_653D4D70B9DE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of Whole Genome (wg-) and Core Genome (cg-) MLST (BioNumerics<sup>TM</sup>) Versus SNP Variant Calling for Epidemiological Investigation of Pseudomonas aeruginosa.
Journal
Frontiers in microbiology
Author(s)
Blanc D.S., Magalhães B., Koenig I., Senn L., Grandbastien B.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
11
Pages
1729
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Whole genome sequencing (WGS) is increasingly used for epidemiological investigations of pathogens. While SNP variant calling is currently considered as the most suitable method, the choice of a representative reference genome and the isolate dependency of results limit standardization and affect resolution in an unknown manner. Whole or core genome Multi Locus Sequence Typing (wg-, cg-MLST) represents an attractive alternative. Here, we assess the accuracy of wg- and cg-MLST by comparing results of four Pseudomonas aeruginosa datasets for which epidemiological and genomic data were previously described. Three datasets included 155 isolates from three different sequence types (ST) of P. aeruginosa collected in our ICUs over a 5-year period. The fourth dataset consisted of 10 isolates from an investigation of P. aeruginosa contaminated hand soap. All isolates were previously analyzed by a core SNP approach. In this study, wg- and cg-MLST were performed in BioNumerics <sup>TM</sup> using a scheme developed by Applied-Maths. Correlation between SNP calling and wg- or cg-MLST results were evaluated by calculating linear regressions and their coefficient of correlations (R <sup>2</sup> ) between the number of SNPs and the number of allele differences in pairwise comparison of isolates. The number of SNPs and allele difference between isolates with close epidemiological linkage varies between 0-26 and 0-13, respectively. When compared to core-SNP calling, a higher coefficient of correlation was obtained with cgMLST (R <sup>2</sup> of 0.92-0.99) than with wgMLST (0.78-0.99). In one dataset, a putative homologous recombination of a large DNA fragment (202 loci) was identified among these isolates, affecting its phylogeny, but with no impact on the epidemiological analysis of outbreak isolates. In conclusion, we showed that the P. aeruginosa wgMLST scheme in BioNumerics <sup>TM</sup> is as discriminatory as the core-SNP calling approach and apparently useful for outbreak investigations. We also showed that epidemiological linked isolates showed less than 26 SNPs or 13 allele differences. These are important figures for the distinction between outbreak and non-outbreak isolates when interpreting WGS results. However, as P. aeruginosa is highly recombinant, a cgMLST approach is preferable and caution should be addressed to possible recombination of large DNA fragments.
Keywords
Pseudomonas aeruginosa, cgMLST, evaluation, molecular typing, wgMLST, whole genome sequencing
Pubmed
Web of science
Open Access
Yes
Create date
15/09/2020 11:43
Last modification date
09/10/2020 6:25
Usage data