Acute brain death abolishes the cardioprotective effects of ischemic preconditioning in the rabbit.

Details

Serval ID
serval:BIB_653454E7D60A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Acute brain death abolishes the cardioprotective effects of ischemic preconditioning in the rabbit.
Journal
Transplantation
Author(s)
Kirsch M., Farhat F., Garnier J.P., Loisance D.
ISSN
0041-1337 (Print)
ISSN-L
0041-1337
Publication state
Published
Issued date
27/05/2000
Peer-reviewed
Oui
Volume
69
Number
10
Pages
2013-2019
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
Myocardial preconditioning with brief coronary artery occlusions before a sustained ischemic period is reported to reduce infarct size. We wished to evaluate whether ischemic preconditioning (IP) is efficient in an experimental brain death (BD) model in the rabbit.
Rabbits were randomized into four experimental groups of eight animals each. In the control group (CTRL), anaesthetized rabbits were subjected to 30 min of left coronary marginal branch occlusion and 90 min of reperfusion without any pretreatment. In the CTRL+IP group, anaesthetized rabbits were preconditioned with a 3-min ischemia and 3-min reperfusion sequence before coronary occlusion. In the BD group, rabbits were subjected to 90 min of BD before 30 min of coronary occlusion and 90 min of reperfusion. In the BD+IP group, BD rabbits were preconditioned as in the CTRL+IP group before coronary occlusion. BD was induced by rapid inflation of an intracranial balloon and was validated by clinical and electroencephalographic examinations. At the termination of the experiment, left ventricular volume (LVV), myocardial volume at risk (VAR) and infarct volume (IV) were determined with methylene blue and tetrazolium staining and were measured using planimetry.
LW was not significantly different among the four experimental groups (CTRL, 6.54+/-0.90 cm3; CTRL+IP, 5.92+/-0.60 cm3; BD, 5.87+/-0.81 cm3; BD+IP, 6.16+/-0.95 cm3; P=ns). Furthermore, myocardial VAR, expressed as a percentage of LVV, was not significantly different between groups (CTRL, 20.0+/-4.2%; CTRL+IP, 22.32+/-2.25%; BD, 21.38+/-3.36%; BD+IP, 21.64+/-3.39%; P=NS). IV, expressed as a percentage of VAR, was significantly reduced in the CTRL+IP group compared with the CTRL group (15.76+/-8.47% vs. 49.95+/-1.51%; P<0.0001). In contrast, there was no significant difference in IV, expressed as a percentage of VAR, between the BD and the BD+IP groups (50.0+/-1.52% vs. 49.72+/-1.58%; P=NS).
The data indicate that the infarct-limiting effect of IP is lost in BD rabbits. Thus, the clinical potential of IP in the context of organ transplantation seems to be severely compromised.
Keywords
Analysis of Variance, Animals, Blood Pressure, Brain Death, Coronary Vessels/physiology, Epinephrine/blood, Heart Rate, Hemodynamics, Ischemic Preconditioning, Myocardial, Myocardial Infarction/pathology, Myocardial Infarction/physiopathology, Myocardial Infarction/prevention & control, Myocardium/pathology, Norepinephrine/blood, Rabbits, Time Factors, Ventricular Function, Left
Pubmed
Web of science
Create date
30/03/2019 17:08
Last modification date
20/08/2019 14:21
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