Efficient gene transfer into mammalian cells with cholesteryl-spermidine

Details

Serval ID
serval:BIB_650FCDFF70AA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Efficient gene transfer into mammalian cells with cholesteryl-spermidine
Journal
Biochemical and Biophysical Research Communications
Author(s)
Moradpour  D., Schauer  J. I., Zurawski, V. R., Jr. , Wands  J. R., Boutin  R. H.
ISSN
0006-291X (Print)
Publication state
Published
Issued date
04/1996
Volume
221
Number
1
Pages
82-8
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 5
Abstract
The naturally occurring polyamine spermidine was covalently conjugated with cholesterol, resulting in a novel cationic compound that mediates efficient gene transfer into mammalian cells. Using reporter plasmids coding for firefly luciferase and beta-galactosidase, a simple procedure was developed allowing highly reproducible and efficient transient and stable transfection of HuH-7 cells. Transfection efficiency could be further increased when a fusogenic peptide derived from the influenza virus hemagglutinin HA2 aminoterminal sequence was included in the cholesteryl-spermidine-DNA complex. Cholesteryl-spermidine (Transfectall) represents a novel cationic compound for efficient transfection of cultured cells in vitro and has the potential to be used for gene transfer in vivo.
Keywords
3T3 Cells Amino Acid Sequence Animals Cell Line, Transformed Cholesterol/chemistry/*metabolism *Gene Transfer Techniques Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/metabolism Humans Mice Molecular Sequence Data Spermidine/chemistry/*metabolism Transfection Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 16:06
Last modification date
20/08/2019 14:21
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