BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance.

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State: Public
Version: Final published version
Serval ID
serval:BIB_64FE7DD6C3CA
Type
Article: article from journal or magazin.
Collection
Publications
Title
BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance.
Journal
eLife
Author(s)
Zhang B., Holmlund M., Lorrain S., Norberg M., Bakó L., Fankhauser C., Nilsson O.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Publication state
Published
Issued date
22/08/2017
Peer-reviewed
Oui
Volume
6
Pages
e26759
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Both light and temperature have dramatic effects on plant development. Phytochrome photoreceptors regulate plant responses to the environment in large part by controlling the abundance of PHYTOCHROME INTERACTING FACTOR (PIF) transcription factors. However, the molecular determinants of this essential signaling mechanism still remain largely unknown. Here, we present evidence that the <i>BLADE-ON-PETIOLE</i> ( <i>BOP</i> ) genes, which have previously been shown to control leaf and flower development in Arabidopsis, are involved in controlling the abundance of PIF4. Genetic analysis shows that <i>BOP2</i> promotes photo-morphogenesis and modulates thermomorphogenesis by suppressing <i>PIF4</i> activity, through a reduction in PIF4 protein level. In red-light-grown seedlings PIF4 ubiquitination was reduced in the <i>bop2</i> mutant. Moreover, we found that BOP proteins physically interact with both PIF4 and CULLIN3A and that a CULLIN3-BOP2 complex ubiquitinates PIF4 in vitro. This shows that BOP proteins act as substrate adaptors in a CUL3 <sup>BOP1/BOP2</sup> E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation.
Keywords
Arabidopsis/growth & development, Arabidopsis/radiation effects, Arabidopsis Proteins/metabolism, Basic Helix-Loop-Helix Transcription Factors/metabolism, Carrier Proteins/metabolism, Gene Expression Regulation, Plant, Light, Plant Development/radiation effects, Protein Binding, Temperature, Ubiquitin-Protein Ligases/metabolism, Ubiquitination, A. thaliana, BOP, E3 ligase, PHYB, PIF4, light signalling, plant biology, ubiquitination
Pubmed
Web of science
Open Access
Yes
Create date
19/09/2017 14:31
Last modification date
20/08/2019 15:21
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