Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.

Details

Ressource 1Download: 28705464_BIB_64ADA65D830A.pdf (1040.82 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_64ADA65D830A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.
Journal
EBioMedicine
Author(s)
Alanio A., Gits-Muselli M., Guigue N., Desnos-Ollivier M., Calderon E.J., Di Cave D., Dupont D., Hamprecht A., Hauser P.M., Helweg-Larsen J., Kicia M., Lagrou K., Lengerova M., Matos O., Melchers WJG, Morio F., Nevez G., Totet A., White L.P., Bretagne S.
ISSN
2352-3964 (Electronic)
ISSN-L
2352-3964
Publication state
Published
Issued date
08/2017
Peer-reviewed
Oui
Volume
22
Pages
155-163
Language
english
Notes
Publication types: Journal Article ; Multicenter Study ; Observational Study
Publication Status: ppublish
Abstract
Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting >500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p<0.001). The distribution of the alleles was significantly different (p<0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p<0.001) and Gt16 with Belgium (5/5, p<0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients.

Keywords
Adult, Aged, DNA, Fungal/genetics, Europe, Female, Genetic Variation, Genotyping Techniques/methods, Humans, Male, Microsatellite Repeats, Middle Aged, Phylogeny, Phylogeography, Pneumocystis carinii/classification, Pneumocystis carinii/genetics, Pneumocystis carinii/isolation & purification, Pneumonia, Pneumocystis/microbiology, Genotyping, MLS typing, Microsatellites, Mixed infection, Pneumocystis jirovecii, Transmission
Pubmed
Web of science
Open Access
Yes
Create date
18/08/2017 14:28
Last modification date
20/08/2019 14:20
Usage data