Ligand-mediated retargeting of recombinant adenovirus for gene transfer in vivo

Details

Serval ID
serval:BIB_64ABA8DC2494
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ligand-mediated retargeting of recombinant adenovirus for gene transfer in vivo
Journal
Gene Therapy
Author(s)
Thoma  C., Wieland  S., Moradpour  D., von Weizsacker  F., Offensperger  S., Madon  J., Blum  H. E., Offensperger  W. B.
ISSN
0969-7128 (Print)
Publication state
Published
Issued date
06/2000
Volume
7
Number
12
Pages
1039-45
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The development of efficient and safe methods for in vivo gene transfer is central to the success of gene therapy. Recombinant adenoviral vectors, although highly efficient, are limited by the host immune response, potential safety hazards due to obligatory cotransfer of viral proteins, and their broad tissue tropism. Here, we demonstrate in an animal model that host range and tissue tropism of a recombinant adenovirus from a distant species can be modified by complexing adenovirus with a cell-specific ligand. Thus, a replication-deficient lacZ recombinant human adenovirus, which naturally does not infect avian cells, allowed highly efficient and specific gene transfer to the liver of ducks in vivo when complexed with N-acetylglucosamine, a ligand for the chicken hepatic lectin. This combination of ligand-mediated receptor targeting with adenoviral uptake and intracellular processing of a given gene represents a novel approach to gene therapy of inherited and acquired liver diseases.
Keywords
Acetylglucosamine/metabolism Adenoviridae/*genetics Animals Ducks Gene Targeting/*methods *Gene Transfer Techniques Humans Lectins/metabolism Ligands Liver/cytology Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 16:06
Last modification date
20/08/2019 14:20
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