The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens.

Details

Serval ID
serval:BIB_644E639A8682
Type
Article: article from journal or magazin.
Publication sub-type
Letter (letter): Communication to the publisher.
Collection
Publications
Title
The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens.
Journal
Plant Signaling and Behavior
Author(s)
Saidi Y., Schaefer D.G., Goloubinoff P., Zrÿd J.P., Finka A.
ISSN
1559-2324[electronic], 1559-2316[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
4
Number
5
Pages
457-459
Language
english
Notes
Publication types: Comment ; Journal Article
Publication Status: ppublish
Abstract
The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in dark grown old moss cells and complete lack of labelling in newly differentiated cells. Furthermore, we demonstrate that stable moss lines harbouring a resistance cassette driven by the CaMV 35S are unable to grow in darkness in the presence of the antibiotic. In contrast to the CaMV 35S, the heat inducible promoter, hsp17.3B showed uniform expression pattern in all cells and tissues following a mild heat shock.
Keywords
F-actin, constitutive/inducible promoter, heat shock, hsp17.3B, sodium salicylate
Pubmed
Open Access
Yes
Create date
23/11/2009 14:18
Last modification date
20/08/2019 15:20
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