Clock-dependent chromatin topology modulates circadian transcription and behavior.

Details

Ressource 1Download: Genes Dev.-2018-Mermet-347-58.pdf (6352.49 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_643E20D79827
Type
Article: article from journal or magazin.
Collection
Publications
Title
Clock-dependent chromatin topology modulates circadian transcription and behavior.
Journal
Genes & development
Author(s)
Mermet J., Yeung J., Hurni C., Mauvoisin D., Gustafson K., Jouffe C., Nicolas D., Emmenegger Y., Gobet C., Franken P., Gachon F., Naef F.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Publication state
Published
Issued date
01/03/2018
Peer-reviewed
Oui
Volume
32
Number
5-6
Pages
347-358
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The circadian clock in animals orchestrates widespread oscillatory gene expression programs, which underlie 24-h rhythms in behavior and physiology. Several studies have shown the possible roles of transcription factors and chromatin marks in controlling cyclic gene expression. However, how daily active enhancers modulate rhythmic gene transcription in mammalian tissues is not known. Using circular chromosome conformation capture (4C) combined with sequencing (4C-seq), we discovered oscillatory promoter-enhancer interactions along the 24-h cycle in the mouse liver and kidney. Rhythms in chromatin interactions were abolished in arrhythmic <i>Bmal1</i> knockout mice. Deleting a contacted intronic enhancer element in the <i>Cryptochrome 1</i> ( <i>Cry1</i> ) gene was sufficient to compromise the rhythmic chromatin contacts in tissues. Moreover, the deletion reduced the daily dynamics of <i>Cry1</i> transcriptional burst frequency and, remarkably, shortened the circadian period of locomotor activity rhythms. Our results establish oscillating and clock-controlled promoter-enhancer looping as a regulatory layer underlying circadian transcription and behavior.
Keywords
Animals, CLOCK Proteins/genetics, Chromatin/genetics, Chromatin/metabolism, Circadian Rhythm/genetics, Cryptochromes/genetics, Cryptochromes/metabolism, Enhancer Elements, Genetic/genetics, Kidney/physiology, Liver/physiology, Mice, Mice, Knockout, Promoter Regions, Genetic/physiology, Sequence Deletion/genetics, Transcription, Genetic/genetics, DNA regulatory elements, chromatin topology, circadian rhythms, promoter–enhancer loops, transcriptional bursting
Pubmed
Web of science
Open Access
Yes
Create date
29/03/2018 19:24
Last modification date
20/08/2019 15:20
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