Protein targeting to glycogen mRNA expression is stimulated by noradrenaline in mouse cortical astrocytes

Details

Serval ID
serval:BIB_63D06C3DD846
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Protein targeting to glycogen mRNA expression is stimulated by noradrenaline in mouse cortical astrocytes
Journal
Glia
Author(s)
Allaman  I., Pellerin  L., Magistretti  P. J.
ISSN
0894-1491 (Print)
Publication state
Published
Issued date
06/2000
Volume
30
Number
4
Pages
382-91
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Brain glycogen levels are dynamically regulated by certain neurotransmitters, including noradrenaline (NA) and vasoactive intestinal peptide (VIP). In particular, glycogen synthesis involves activation by NA and VIP of the transcription factors C/EBPbeta and -delta as well as the induction of glycogen synthase. Glycogen accumulation is found in a variety of neuropathological conditions, including reactive astrocytosis after CNS lesions, as well as in Alzheimer's disease. Protein targeting to glycogen (PTG) belongs to a family of proteins that play a key role in glycogen synthesis in peripheral tissues. In this study, we report the presence of PTG mRNA in adult mouse brain, as well as in astrocytes, a non-neuronal cell type that contains most of brain glycogen. Using primary cultures of mouse cortical astrocytes, we observed that NA leads to time- and concentration-dependent induction of PTG mRNA expression. This effect, concomitant to an enhancement of glycogen synthesis in these cells, depends on the activation of beta(1)-adrenergic receptors. Induction of PTG mRNA expression was mimicked by the adenylate cyclase activator forskolin and by dibutyryl cAMP, suggesting the involvement of the cAMP-dependent signal transduction cascade. Among other neuroactive substances known to elevate glycogen levels in astrocytes, VIP had a comparable effect to that of noradrenaline, whereas insulin and glutamate were without effect on PTG mRNA expression. These data suggest that increased PTG expression by neurotransmitters such as noradrenaline and VIP could represent a major event leading to enhancement of glycogen levels in astrocytes.
Keywords
Animals Astrocytes/cytology/drug effects/*metabolism Bucladesine/pharmacology Carrier Proteins/genetics/*metabolism Cells, Cultured Cerebral Cortex/cytology/drug effects/*metabolism Forskolin/pharmacology Gene Expression/drug effects/*physiology Glutamic Acid/pharmacology Glycogen/biosynthesis/*genetics Insulin/pharmacology *Intracellular Signaling Peptides and Proteins Mice Norepinephrine/*metabolism/pharmacology RNA, Messenger/*biosynthesis Receptors, Adrenergic, beta-1/drug effects Vasoactive Intestinal Peptide/metabolism/pharmacology
Pubmed
Web of science
Create date
24/01/2008 13:17
Last modification date
20/08/2019 14:20
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