Retroviral DNA integration: HIV and the role of LEDGF/p75

Details

Serval ID
serval:BIB_63CF8A34898A
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Retroviral DNA integration: HIV and the role of LEDGF/p75
Journal
Trends in Genetics
Author(s)
Ciuffi  A., Bushman  F. D.
ISSN
0168-9525
Publication state
Published
Issued date
07/2006
Peer-reviewed
Oui
Volume
22
Number
7
Pages
388-95
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review --- Old month value: Jul
Abstract
To replicate, a retrovirus must integrate a DNA copy of its RNA genome into a chromosome of the host cell. Integration is not random in the host genome but favors particular regions, and preferences differ among retroviruses. Several mechanisms might play a part in this favored integration targeting: (i) open chromatin might be preferentially accessible for viral DNA integration; (ii) DNA replication during cell division might facilitate access of integration complexes to favored sites; and (iii) cellular proteins bound to the host chromosome might tether integration complexes to favored regions. This review summarizes recent advances in understanding the mechanisms of retroviral integration, focusing on LEDGF/p75--the first cellular protein shown to have a role in directing HIV DNA integration. Studies on LEDGF/p75 indicate that it directs HIV integration site selection by a tethering interaction, whereas the chromatin accessibility or cell cycle models are less well supported. Understanding viral integration will help improve the safety of retrovirus-based vectors used in gene therapy.
Keywords
Chromatin/metabolism DNA, Viral/*physiology HIV/*physiology HIV Infections/metabolism/virology Humans Integrases/metabolism Intercellular Signaling Peptides and Proteins/*physiology Virus Integration/*physiology Virus Replication
Pubmed
Web of science
Create date
22/02/2008 14:45
Last modification date
20/08/2019 14:20
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